Gene description for HERC4
Gene name HECT and RLD domain containing E3 ubiquitin protein ligase 4
Gene symbol HERC4
Other names/aliases -
Species Homo sapiens
 Database cross references - HERC4
ExoCarta ExoCarta_26091
Vesiclepedia VP_26091
Entrez Gene 26091
HGNC 24521
MIM 609248
UniProt Q5GLZ8  
 HERC4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HERC4
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    spermatogenesis GO:0007283 ISS
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    cell differentiation GO:0030154 IEA
    negative regulation of smoothened signaling pathway GO:0045879 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified HERC4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HERC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
2 BAG3 9531
Affinity Capture-MS Homo sapiens
3 FDXACB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ANKRD39  
Affinity Capture-MS Homo sapiens
5 Esrrb  
Affinity Capture-MS Mus musculus
6 FAM189B  
Affinity Capture-MS Homo sapiens
7 UBE2J2 118424
Reconstituted Complex Homo sapiens
8 ISG15 9636
Affinity Capture-MS Homo sapiens
9 NUF2  
Affinity Capture-MS Homo sapiens
10 Lin7c 22343
Affinity Capture-MS Mus musculus
11 GMNN  
Affinity Capture-MS Homo sapiens
12 PRKY  
Affinity Capture-MS Homo sapiens
13 NXF1 10482
Affinity Capture-RNA Homo sapiens
14 RAD23A 5886
Co-fractionation Homo sapiens
15 ABHD15  
Affinity Capture-MS Homo sapiens
16 ALOXE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TPT1 7178
Co-fractionation Homo sapiens
18 APOBEC3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RB1 5925
Affinity Capture-MS Homo sapiens
20 USP5 8078
Co-fractionation Homo sapiens
21 APEX1 328
Affinity Capture-RNA Homo sapiens
22 CARD16  
Affinity Capture-MS Homo sapiens
23 NUBP2 10101
Co-fractionation Homo sapiens
24 C9orf78 51759
Co-fractionation Homo sapiens
25 ARSH  
Affinity Capture-MS Homo sapiens
26 UBC 7316
Biochemical Activity Homo sapiens
27 FKBP9 11328
Co-fractionation Homo sapiens
28 PGR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 WRAP73 49856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
31 SHMT2 6472
Affinity Capture-RNA Homo sapiens
32 PLD5  
Affinity Capture-MS Homo sapiens
33 Ppp6c 67857
Affinity Capture-MS Mus musculus
34 NLRX1 79671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HEXA 3073
Co-fractionation Homo sapiens
36 PCNA 5111
Co-fractionation Homo sapiens
37 NT5C2 22978
Co-fractionation Homo sapiens
38 ERCC3  
Co-fractionation Homo sapiens
39 UBE2D2 7322
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 HERC6  
Affinity Capture-Western Homo sapiens
42 MAF  
Affinity Capture-MS Homo sapiens
43 WDR72  
Affinity Capture-MS Homo sapiens
44 GNB2 2783
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
47 C1orf94  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 H2AFV 94239
Co-fractionation Homo sapiens
49 ZNF215  
Affinity Capture-MS Homo sapiens
50 SMO 6608
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 LSM6 11157
Co-fractionation Homo sapiens
52 KLHL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 FLII 2314
Affinity Capture-MS Homo sapiens
54 HERC4 26091
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
55 MOCS3 27304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 UBE2E3 10477
Reconstituted Complex Homo sapiens
57 WARS 7453
Co-fractionation Homo sapiens
58 INF2 64423
Affinity Capture-MS Homo sapiens
59 PLD2 5338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 Katna1  
Affinity Capture-MS Mus musculus
61 DCTPP1 79077
Co-fractionation Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
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