Gene description for Map4k4
Gene name mitogen-activated protein kinase kinase kinase kinase 4
Gene symbol Map4k4
Other names/aliases 9430080K19Rik
AU043147
AU045934
AW046177
HGK
Nik
Species Mus musculus
 Database cross references - Map4k4
ExoCarta ExoCarta_26921
Vesiclepedia VP_26921
Entrez Gene 26921
UniProt P97820  
 Map4k4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Map4k4
Molecular Function
    creatine kinase activity GO:0004111 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 ISO
    microtubule binding GO:0008017 ISO
    MAP kinase kinase kinase kinase activity GO:0008349 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of ARF protein signal transduction GO:0032014 IMP
    positive regulation of hippo signaling GO:0035332 ISO
    intracellular signal transduction GO:0035556 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    regulation of MAPK cascade GO:0043408 IBA
    positive regulation of GTPase activity GO:0043547 IMP
    regulation of JNK cascade GO:0046328 ISO
    regulation of JNK cascade GO:0046328 ISS
    positive regulation of JNK cascade GO:0046330 IDA
    neuron projection morphogenesis GO:0048812 IBA
    positive regulation of keratinocyte migration GO:0051549 IMP
    positive regulation of focal adhesion assembly GO:0051894 IMP
    negative regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061179 ISO
    negative regulation of neuron projection regeneration GO:0070571 ISO
    positive regulation of focal adhesion disassembly GO:0120183 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Map4k4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Map4k4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cep290  
Affinity Capture-MS Mus musculus
2 Fxr1 14359
Affinity Capture-MS Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Fmr1  
Affinity Capture-MS Mus musculus
6 Pde4dip  
Affinity Capture-MS Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Shank3  
Affinity Capture-MS Mus musculus
10 Kif26b  
Co-fractionation Mus musculus
11 Mvb12a 73711
Co-fractionation Mus musculus
12 Fry  
Co-fractionation Mus musculus
13 Dlgap1  
Affinity Capture-MS Mus musculus
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