Gene description for Mvb12a
Gene name multivesicular body subunit 12A
Gene symbol Mvb12a
Other names/aliases 1110012M11Rik
Fam125a
Species Mus musculus
 Database cross references - Mvb12a
ExoCarta ExoCarta_73711
Vesiclepedia VP_73711
Entrez Gene 73711
UniProt Q78HU3  
 Mvb12a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mvb12a
Molecular Function
    lipid binding GO:0008289 IEA
    lipid binding GO:0008289 ISO
    SH3 domain binding GO:0017124 IEA
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
Biological Process
    protein transport GO:0015031 IEA
    virus maturation GO:0019075 IBA
    virus maturation GO:0019075 IEA
    virus maturation GO:0019075 ISO
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 IBA
    receptor catabolic process GO:0032801 IBA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IBA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IEA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 ISO
    viral budding GO:0046755 IBA
    viral budding GO:0046755 IEA
    viral budding GO:0046755 ISO
Subcellular Localization
    ESCRT I complex GO:0000813 IBA
    ESCRT I complex GO:0000813 IEA
    ESCRT I complex GO:0000813 ISO
    nucleus GO:0005634 IEA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    late endosome membrane GO:0031902 IEA
    vesicle GO:0031982 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Mvb12a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mvb12a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Map4k4 26921
Co-fractionation Mus musculus
View the network image/svg+xml



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