Gene description for Racgap1
Gene name Rac GTPase-activating protein 1
Gene symbol Racgap1
Other names/aliases AI227039
AI327394
Band25
GTPase
MgcRacGAP
gtl11
mKIAA1478
Species Mus musculus
 Database cross references - Racgap1
ExoCarta ExoCarta_26934
Vesiclepedia VP_26934
Entrez Gene 26934
UniProt Q9WVM1  
 Racgap1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Racgap1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISO
    GTPase activator activity GO:0005096 ISS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    alpha-tubulin binding GO:0043014 ISO
    alpha-tubulin binding GO:0043014 ISS
    gamma-tubulin binding GO:0043015 ISO
    gamma-tubulin binding GO:0043015 ISS
    metal ion binding GO:0046872 IEA
    beta-tubulin binding GO:0048487 ISO
    beta-tubulin binding GO:0048487 ISS
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    actomyosin contractile ring assembly GO:0000915 ISO
    actomyosin contractile ring assembly GO:0000915 ISS
    monoatomic ion transport GO:0006811 IEA
    signal transduction GO:0007165 IEA
    Rho protein signal transduction GO:0007266 IBA
    spermatogenesis GO:0007283 ISS
    neuroblast proliferation GO:0007405 IEP
    erythrocyte differentiation GO:0030218 ISO
    erythrocyte differentiation GO:0030218 ISS
    positive regulation of cytokinesis GO:0032467 ISO
    positive regulation of cytokinesis GO:0032467 ISS
    regulation of embryonic development GO:0045995 IMP
    mitotic spindle midzone assembly GO:0051256 IBA
    mitotic spindle midzone assembly GO:0051256 ISO
    mitotic spindle midzone assembly GO:0051256 ISS
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISO
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISS
    sulfate transmembrane transport GO:1902358 IEA
    sulfate transmembrane transport GO:1902358 ISO
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spindle GO:0005819 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    midbody GO:0030496 IBA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cleavage furrow GO:0032154 IBA
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISS
    spindle midzone GO:0051233 IBA
    spindle midzone GO:0051233 ISO
    spindle midzone GO:0051233 ISS
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    Flemming body GO:0090543 IEA
    centralspindlin complex GO:0097149 IBA
    centralspindlin complex GO:0097149 ISO
    centralspindlin complex GO:0097149 ISS
 Experiment description of studies that identified Racgap1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Racgap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2C 5432
Affinity Capture-MS Homo sapiens
2 Slc26a8  
Co-fractionation Mus musculus
3 Map3k4  
Co-fractionation Mus musculus
4 Pde4dip  
Affinity Capture-MS Mus musculus
5 Gmip  
Co-fractionation Mus musculus
6 MTA2 9219
Affinity Capture-MS Homo sapiens
7 EEA1 8411
Affinity Capture-MS Homo sapiens
8 ASCC1 51008
Affinity Capture-MS Homo sapiens
9 RACGAP1 29127
Affinity Capture-MS Homo sapiens
10 TARDBP 23435
Affinity Capture-MS Homo sapiens
11 DDX3X 1654
Affinity Capture-MS Homo sapiens
12 ACTA2 59
Affinity Capture-MS Homo sapiens
13 RCC2 55920
Affinity Capture-MS Homo sapiens
14 PAK2 5062
Affinity Capture-MS Homo sapiens
15 CD2AP 23607
Affinity Capture-MS Homo sapiens
16 RBM12B  
Affinity Capture-MS Homo sapiens
17 MANF 7873
Affinity Capture-MS Homo sapiens
18 IL24  
Affinity Capture-MS Homo sapiens
19 FBXL19  
Affinity Capture-MS Homo sapiens
20 FAM192A  
Affinity Capture-MS Homo sapiens
21 Rab11fip4  
Co-fractionation Mus musculus
22 Tmem173  
Proximity Label-MS Mus musculus
23 SAFB 6294
Affinity Capture-MS Homo sapiens
24 ANXA11 311
Affinity Capture-MS Homo sapiens
25 PPIL2  
Affinity Capture-MS Homo sapiens
26 KIAA2013 90231
Affinity Capture-MS Homo sapiens
27 CCNL2 81669
Affinity Capture-MS Homo sapiens
28 NRAS 4893
Affinity Capture-MS Homo sapiens
29 MICAL3 57553
Affinity Capture-MS Homo sapiens
30 ESCO1  
Affinity Capture-MS Homo sapiens
31 KIF23 9493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FLNA 2316
Affinity Capture-MS Homo sapiens
33 PHF21A  
Affinity Capture-MS Homo sapiens
34 Zbtb7b  
Affinity Capture-MS Mus musculus
35 PRC1 9055
Affinity Capture-MS Homo sapiens
36 EIF5A 1984
Affinity Capture-MS Homo sapiens
37 NOB1  
Affinity Capture-MS Homo sapiens
38 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
39 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 GAS2L1 10634
Affinity Capture-MS Homo sapiens
41 COPS7B 64708
Affinity Capture-MS Homo sapiens
42 GMPS 8833
Affinity Capture-MS Homo sapiens
43 POLR2G 5436
Affinity Capture-MS Homo sapiens
44 FUBP1 8880
Affinity Capture-MS Homo sapiens
45 MYL12A 10627
Affinity Capture-MS Homo sapiens
46 GPKOW  
Affinity Capture-MS Homo sapiens
47 NAB1 4664
Affinity Capture-MS Homo sapiens
48 ELP3 55140
Affinity Capture-MS Homo sapiens
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