Experiment description of studies that identified HSP90AB3P in sEVs

1 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

2 Experiment ID 1275 MISEV standards Biophysical techniques ✔ CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 1280 MISEV standards Biophysical techniques ✔ TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

5 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for HSP90AB3P

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FTL 2512 Co-fractionation Homo sapiens 2 ANLN 54443 Affinity Capture-MS Homo sapiens 3 TNK2 10188 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 MAP2K2 5605 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 NUAK2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 7 SGK2   Affinity Capture-MS Homo sapiens 8 SPRTN   Affinity Capture-MS Homo sapiens 9 UBL4A 8266 Affinity Capture-MS Homo sapiens 10 CDK7 1022 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 CCDC117   Affinity Capture-MS Homo sapiens 12 KIF14 9928 Affinity Capture-MS Homo sapiens 13 CLK3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 MYLK4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 GRK7   Affinity Capture-MS Homo sapiens 16 CHMP4C 92421 Affinity Capture-MS Homo sapiens 17 CLTA 1211 Affinity Capture-MS Homo sapiens 18 AMHR2   Affinity Capture-MS Homo sapiens 19 RPS6KA4 8986 Affinity Capture-MS Homo sapiens 20 CDC37 11140 Affinity Capture-MS Homo sapiens 21 AURKC   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 CDKL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 HSP90AB1 3326 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 24 CUL3 8452 Affinity Capture-MS Homo sapiens 25 TTC4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 AKT3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 USP11 8237 Affinity Capture-MS Homo sapiens 29 HSP90AA1 3320 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 30 GBP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 SOX2   Affinity Capture-MS Homo sapiens 32 GUCY2D   Affinity Capture-MS Homo sapiens 33 TFCP2 7024 Affinity Capture-MS Homo sapiens 34 AURKB 9212 Affinity Capture-MS Homo sapiens 35 YES1 7525 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 RPA3 6119 Proximity Label-MS Homo sapiens 37 TSSK6 83983 Affinity Capture-MS Homo sapiens 38 CDK13 8621 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 STK35   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 ANKMY2 57037 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 FYN 2534 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 YAP1 10413 Affinity Capture-MS Homo sapiens 43 CDK4 1019 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 FBXL4 26235 Affinity Capture-MS Homo sapiens 45 KIF23 9493 Affinity Capture-MS Homo sapiens 46 CDK9 1025 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 47 PINK1   Affinity Capture-MS Homo sapiens 48 NEK4   Affinity Capture-MS Homo sapiens 49 CDKL4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 PTGES3 10728 Affinity Capture-MS Homo sapiens 51 STK11 6794 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 SMYD3 64754 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 UNC45B   Affinity Capture-MS Homo sapiens 54 AGO3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 CRY2   Affinity Capture-MS Homo sapiens 56 CHMP4B 128866 Affinity Capture-MS Homo sapiens 57 ITK 3702 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 58 SRMS   Affinity Capture-MS Homo sapiens 59 BTK 695 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 CIT 11113 Affinity Capture-MS Homo sapiens 61 INSRR 3645 Affinity Capture-MS Homo sapiens 62 STIP1 10963 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 PLD2 5338 Affinity Capture-MS Homo sapiens 64 CDK11A 728642 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 PRKCE 5581 Affinity Capture-MS Homo sapiens 66 GPAT2   Affinity Capture-MS Homo sapiens 67 VAMP5 10791 Affinity Capture-MS Homo sapiens View the network

Pathways in which HSP90AB3P is involved

No pathways found