Gene description for KCNMA1
Gene name potassium channel, calcium activated large conductance subfamily M alpha, member 1
Gene symbol KCNMA1
Other names/aliases BKTM
KCa1.1
MaxiK
SAKCA
SLO
SLO-ALPHA
SLO1
bA205K10.1
mSLO1
Species Homo sapiens
 Database cross references - KCNMA1
ExoCarta ExoCarta_3778
Vesiclepedia VP_3778
Entrez Gene 3778
HGNC 6284
MIM 600150
UniProt Q12791  
 KCNMA1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KCNMA1
Molecular Function
    actin binding GO:0003779 IDA
    voltage-gated potassium channel activity GO:0005249 IDA
    voltage-gated potassium channel activity GO:0005249 IMP
    protein binding GO:0005515 IPI
    calcium-activated potassium channel activity GO:0015269 IDA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    large conductance calcium-activated potassium channel activity GO:0060072 IBA
    large conductance calcium-activated potassium channel activity GO:0060072 IDA
    large conductance calcium-activated potassium channel activity GO:0060072 IMP
Biological Process
    response to hypoxia GO:0001666 IDA
    potassium ion transport GO:0006813 IDA
    response to osmotic stress GO:0006970 IDA
    intracellular potassium ion homeostasis GO:0030007 IDA
    response to carbon monoxide GO:0034465 IDA
    response to carbon monoxide GO:0034465 IMP
    vasodilation GO:0042311 IBA
    regulation of membrane potential GO:0042391 IBA
    regulation of membrane potential GO:0042391 IDA
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of cell volume GO:0045794 IDA
    response to calcium ion GO:0051592 IDA
    micturition GO:0060073 IDA
    smooth muscle contraction involved in micturition GO:0060083 IDA
    potassium ion transmembrane transport GO:0071805 IBA
    potassium ion transmembrane transport GO:0071805 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IDA
    voltage-gated potassium channel complex GO:0008076 IDA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IDA
    postsynaptic membrane GO:0045211 IBA
 Experiment description of studies that identified KCNMA1 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KCNMA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TBXA2R 6915
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
2 PRC1 9055
Affinity Capture-MS Homo sapiens
3 ALB 213
Affinity Capture-MS Homo sapiens
4 PTPRA 5786
Affinity Capture-MS Homo sapiens
5 KCNMA1 3778
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 KCNMB1  
Affinity Capture-Western Homo sapiens
7 EED  
Affinity Capture-MS Homo sapiens
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