Gene description for MPL
Gene name MPL proto-oncogene, thrombopoietin receptor
Gene symbol MPL
Other names/aliases C-MPL
CD110
MPLV
THCYT2
TPOR
Species Homo sapiens
 Database cross references - MPL
ExoCarta ExoCarta_4352
Vesiclepedia VP_4352
Entrez Gene 4352
HGNC 7217
MIM 159530
UniProt P40238  
 MPL identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MPL
Molecular Function
    protein binding GO:0005515 IPI
    thrombopoietin receptor activity GO:0038164 IBA
    thrombopoietin receptor activity GO:0038164 IMP
Biological Process
    neutrophil homeostasis GO:0001780 IEA
    monocyte homeostasis GO:0035702 IEA
    thrombopoietin-mediated signaling pathway GO:0038163 IBA
    thrombopoietin-mediated signaling pathway GO:0038163 IDA
    positive regulation of lymphocyte proliferation GO:0050671 IEA
    cellular response to hypoxia GO:0071456 IEA
    positive regulation of platelet formation GO:1905221 IEA
    eosinophil homeostasis GO:1990959 IEA
    basophil homeostasis GO:1990960 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    nuclear membrane GO:0031965 IDA
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified MPL in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF282  
Affinity Capture-MS Homo sapiens
2 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
3 PDXP 57026
Affinity Capture-MS Homo sapiens
4 ALX1  
Affinity Capture-MS Homo sapiens
5 ANKRA2  
Protein-peptide Homo sapiens
6 ALCAM 214
Affinity Capture-MS Homo sapiens
7 TSC2 7249
Affinity Capture-MS Homo sapiens
8 ACOT9 23597
Affinity Capture-MS Homo sapiens
9 IGFBP5 3488
Affinity Capture-MS Homo sapiens
10 CTDNEP1 23399
Affinity Capture-MS Homo sapiens
11 EIF2AK3  
Affinity Capture-MS Homo sapiens
12 TYW1  
Affinity Capture-MS Homo sapiens
13 ROBO1 6091
Affinity Capture-MS Homo sapiens
14 SOCS1  
Affinity Capture-Western Homo sapiens
15 EGFR 1956
Affinity Capture-MS Homo sapiens
16 ADGRL3 23284
Affinity Capture-MS Homo sapiens
17 LRP12 29967
Affinity Capture-MS Homo sapiens
18 NSUN3  
Affinity Capture-MS Homo sapiens
19 BTN2A2  
Affinity Capture-MS Homo sapiens
20 GOLGA7 51125
Affinity Capture-MS Homo sapiens
21 ERCC6  
Affinity Capture-MS Homo sapiens
22 RETSAT 54884
Affinity Capture-MS Homo sapiens
23 CCDC51 79714
Affinity Capture-MS Homo sapiens
24 TYK2 7297
Affinity Capture-MS Homo sapiens
25 FAM171A2 284069
Affinity Capture-MS Homo sapiens
26 KCNT2  
Affinity Capture-MS Homo sapiens
27 ATXN2L 11273
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
28 C17orf70  
Affinity Capture-MS Homo sapiens
29 YWHAQ 10971
Protein-peptide Homo sapiens
30 CISD2 493856
Affinity Capture-MS Homo sapiens
31 CTH 1491
Affinity Capture-MS Homo sapiens
32 PAX3  
Affinity Capture-MS Homo sapiens
33 ATP2A3 489
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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