Gene ontology annotations for NME1
Experiment description of studies that identified NME1 in sEVs
1
Experiment ID
76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
2
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
6
Experiment ID
679
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-231T
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
7
Experiment ID
680
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-231T
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
8
Experiment ID
681
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-435
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
682
MISEV standards
✔
EM
Biophysical techniques
✔
FLOT1|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-435
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
10
Experiment ID
683
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
T47D
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
11
Experiment ID
684
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MCF-7
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
12
Experiment ID
685
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-453
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
13
Experiment ID
686
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-436
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
14
Experiment ID
687
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
MDA-MB-468
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
15
Experiment ID
688
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
BT-474
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
16
Experiment ID
689
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
BT549
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
17
Experiment ID
690
MISEV standards
✘
Biophysical techniques
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
35939247
Organism
Homo sapiens
Experiment description
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems
Authors
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS."
Journal name
Clin Exp Metastasis
Publication year
2022
Sample
Breast cancer cells
Sample name
JIMT1-BR
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
18
Experiment ID
20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
19
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
20
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
21
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
22
Experiment ID
236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
23
Experiment ID
237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
24
Experiment ID
255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
G1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
501mel
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
Daju
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
27
Experiment ID
258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
30
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
31
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
32
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
33
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
34
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
35
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
36
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
38
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
39
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
40
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
41
Experiment ID
66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
42
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for NME1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ITGB1
3688
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
2
NME2P1
283458
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
3
GATA1
Affinity Capture-Western
Homo sapiens
4
UBE2H
7328
Affinity Capture-MS
Homo sapiens
5
OXCT1
5019
Co-fractionation
Homo sapiens
6
AP2B1
163
Co-fractionation
Homo sapiens
7
IDH1
3417
Co-fractionation
Homo sapiens
8
RIN3
Affinity Capture-MS
Homo sapiens
9
RRAD
6236
Affinity Capture-Western
Homo sapiens
10
EYA2
Affinity Capture-MS
Homo sapiens
11
CFL1
1072
Co-fractionation
Homo sapiens
12
ASH1L-AS1
Affinity Capture-RNA
Homo sapiens
13
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
14
CHRAC1
Co-fractionation
Homo sapiens
15
IGF2BP3
10643
Affinity Capture-MS
Homo sapiens
16
MCM2
4171
Affinity Capture-MS
Homo sapiens
17
USP18
Affinity Capture-MS
Homo sapiens
18
SMYD3
64754
Affinity Capture-MS
Homo sapiens
19
CALD1
800
Co-fractionation
Homo sapiens
20
DUSP2
Affinity Capture-MS
Homo sapiens
21
MAPK14
1432
Co-fractionation
Homo sapiens
22
MARCH5
Proximity Label-MS
Homo sapiens
23
TIAM1
Affinity Capture-Western
Homo sapiens
24
DYNLL1
8655
Co-fractionation
Homo sapiens
25
FANCD2
Affinity Capture-MS
Homo sapiens
26
SOX30
Two-hybrid
Homo sapiens
27
NME2
4831
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
28
MECP2
4204
Affinity Capture-MS
Homo sapiens
29
MYCN
Affinity Capture-MS
Homo sapiens
30
SOD1
6647
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
31
WDR1
9948
Co-fractionation
Homo sapiens
32
KLF16
Affinity Capture-MS
Homo sapiens
33
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
34
AURKA
6790
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
35
PRPF6
24148
Two-hybrid
Homo sapiens
36
DLD
1738
Co-fractionation
Homo sapiens
37
RBBP7
5931
Co-fractionation
Homo sapiens
38
PRDX5
25824
Co-fractionation
Homo sapiens
39
PKM
5315
Co-fractionation
Homo sapiens
40
TNPO2
30000
Two-hybrid
Homo sapiens
41
FUBP1
8880
Co-fractionation
Homo sapiens
42
VCP
7415
Affinity Capture-MS
Homo sapiens
43
GORASP2
26003
Two-hybrid
Homo sapiens
44
EYA3
Affinity Capture-MS
Homo sapiens
45
NME3
4832
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
46
Ube2i
Two-hybrid
Mus musculus
47
EBNA-LP
Affinity Capture-MS
48
HDAC5
10014
Affinity Capture-MS
Homo sapiens
49
DNM1L
10059
Affinity Capture-MS
Homo sapiens
50
ASB18
Affinity Capture-MS
Homo sapiens
51
WDYHV1
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
52
PID1
55022
Two-hybrid
Homo sapiens
53
DUSP22
Affinity Capture-MS
Homo sapiens
54
PER2
Proximity Label-MS
Homo sapiens
55
AHCY
191
Co-fractionation
Homo sapiens
56
VAPB
9217
Affinity Capture-MS
Homo sapiens
57
SMARCD1
6602
Two-hybrid
Homo sapiens
58
NME1-NME2
654364
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
59
PRDX1
5052
Co-fractionation
Homo sapiens
60
CUL3
8452
Affinity Capture-MS
Homo sapiens
61
NUP43
348995
Proximity Label-MS
Homo sapiens
62
FBXO6
26270
Affinity Capture-MS
Homo sapiens
63
PRUNE
58497
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
64
SORD
6652
Co-fractionation
Homo sapiens
65
OCIAD1
54940
Proximity Label-MS
Homo sapiens
66
NXF1
10482
Two-hybrid
Homo sapiens
67
STRAP
11171
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
68
SOAT1
6646
Affinity Capture-MS
Homo sapiens
69
MYC
Affinity Capture-MS
Homo sapiens
70
BAG1
573
Affinity Capture-MS
Homo sapiens
71
NME1
4830
Two-hybrid
Homo sapiens
Co-crystal Structure
Homo sapiens
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Co-crystal Structure
Homo sapiens
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
72
FDPS
2224
Co-fractionation
Homo sapiens
73
SET
6418
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
74
FBXO24
26261
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
75
BMI1
Affinity Capture-MS
Homo sapiens
76
MSN
4478
Co-fractionation
Homo sapiens
77
FOXP4
Affinity Capture-MS
Homo sapiens
78
HNRNPH1
3187
Affinity Capture-RNA
Homo sapiens
79
AKAP1
8165
Proximity Label-MS
Homo sapiens
80
UROD
7389
Co-fractionation
Homo sapiens
81
DUSP19
Affinity Capture-MS
Homo sapiens
82
NUTF2
10204
Two-hybrid
Homo sapiens
83
KAT2A
Affinity Capture-Western
Homo sapiens
84
JUP
3728
Affinity Capture-MS
Homo sapiens
85
TUBG1
7283
Affinity Capture-MS
Homo sapiens
86
NME4
4833
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
87
POLR1C
9533
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
88
THOP1
7064
Co-fractionation
Homo sapiens
89
CTSD
1509
Co-fractionation
Homo sapiens
90
WTAP
9589
Affinity Capture-MS
Homo sapiens
91
DDX19A
55308
Two-hybrid
Homo sapiens
92
MIF
4282
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
93
TERF1
7013
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
94
NQO1
1728
Co-fractionation
Homo sapiens
95
CRIP1
1396
Co-fractionation
Homo sapiens
96
SREK1IP1
Two-hybrid
Homo sapiens
97
PINK1
Affinity Capture-MS
Homo sapiens
98
GOT1
2805
Co-fractionation
Homo sapiens
99
ERG
Affinity Capture-MS
Homo sapiens
100
C9orf72
Affinity Capture-MS
Homo sapiens
101
RAC1
5879
Biochemical Activity
Homo sapiens
102
ATXN3
4287
Affinity Capture-MS
Homo sapiens
103
WARS
7453
Co-fractionation
Homo sapiens
104
ALDOA
226
Co-fractionation
Homo sapiens
105
HNRNPA2B1
3181
Co-fractionation
Homo sapiens
106
CCND3
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which NME1 is involved