Gene description for PKD2
Gene name polycystic kidney disease 2 (autosomal dominant)
Gene symbol PKD2
Other names/aliases APKD2
PC2
PKD4
Pc-2
TRPP2
Species Homo sapiens
 Database cross references - PKD2
ExoCarta ExoCarta_5311
Vesiclepedia VP_5311
Entrez Gene 5311
HGNC 9009
MIM 173910
UniProt Q13563  
 PKD2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for PKD2
Molecular Function
    signaling receptor binding GO:0005102 IBA
    signaling receptor binding GO:0005102 IPI
    voltage-gated monoatomic ion channel activity GO:0005244 IDA
    voltage-gated calcium channel activity GO:0005245 IBA
    voltage-gated calcium channel activity GO:0005245 IDA
    voltage-gated sodium channel activity GO:0005248 IBA
    voltage-gated sodium channel activity GO:0005248 IDA
    voltage-gated sodium channel activity GO:0005248 IMP
    voltage-gated potassium channel activity GO:0005249 IMP
    monoatomic cation channel activity GO:0005261 IMP
    calcium channel activity GO:0005262 IDA
    potassium channel activity GO:0005267 IBA
    potassium channel activity GO:0005267 IDA
    potassium channel activity GO:0005267 ISS
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 IDA
    outward rectifier potassium channel activity GO:0015271 ISS
    voltage-gated monoatomic cation channel activity GO:0022843 IDA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISS
    protein homodimerization activity GO:0042803 IDA
    actinin binding GO:0042805 IDA
    HLH domain binding GO:0043398 IPI
    transmembrane transporter binding GO:0044325 IPI
    calcium-induced calcium release activity GO:0048763 IDA
    ATPase binding GO:0051117 ISS
    phosphoprotein binding GO:0051219 IPI
    muscle alpha-actinin binding GO:0051371 IBA
    transcription regulator inhibitor activity GO:0140416 IPI
Biological Process
    branching involved in ureteric bud morphogenesis GO:0001658 IEP
    liver development GO:0001889 IEP
    embryonic placenta development GO:0001892 ISS
    heart looping GO:0001947 IMP
    detection of nodal flow GO:0003127 ISS
    calcium ion transport GO:0006816 IDA
    calcium ion transport GO:0006816 ISS
    intracellular calcium ion homeostasis GO:0006874 IEA
    cell surface receptor signaling pathway GO:0007166 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 ISO
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 ISS
    determination of left/right symmetry GO:0007368 ISS
    heart development GO:0007507 IEP
    negative regulation of cell population proliferation GO:0008285 NAS
    positive regulation of gene expression GO:0010628 IEA
    Wnt signaling pathway GO:0016055 IDA
    calcium-mediated signaling GO:0019722 IEA
    spinal cord development GO:0021510 IEP
    neural tube development GO:0021915 IEP
    positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0031587 IMP
    cellular response to reactive oxygen species GO:0034614 NAS
    metanephric part of ureteric bud development GO:0035502 IEP
    sodium ion transmembrane transport GO:0035725 IDA
    sodium ion transmembrane transport GO:0035725 IMP
    aorta development GO:0035904 IEP
    regulation of cell population proliferation GO:0042127 IMP
    cilium organization GO:0044782 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    detection of mechanical stimulus GO:0050982 IBA
    detection of mechanical stimulus GO:0050982 ISS
    release of sequestered calcium ion into cytosol GO:0051209 IBA
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    release of sequestered calcium ion into cytosol GO:0051209 IMP
    protein tetramerization GO:0051262 IDA
    protein homotetramerization GO:0051289 IDA
    protein heterotetramerization GO:0051290 IDA
    centrosome duplication GO:0051298 NAS
    establishment of localization in cell GO:0051649 IEA
    regulation of cell cycle GO:0051726 ISS
    placenta blood vessel development GO:0060674 ISS
    renal tubule morphogenesis GO:0061333 ISS
    renal artery morphogenesis GO:0061441 IEP
    calcium ion transmembrane transport GO:0070588 IDA
    cellular response to calcium ion GO:0071277 ISS
    cellular response to cAMP GO:0071320 IMP
    cellular response to hydrostatic pressure GO:0071464 IDA
    cellular response to osmotic stress GO:0071470 IDA
    cellular response to fluid shear stress GO:0071498 IMP
    potassium ion transmembrane transport GO:0071805 IBA
    potassium ion transmembrane transport GO:0071805 IDA
    potassium ion transmembrane transport GO:0071805 IMP
    potassium ion transmembrane transport GO:0071805 ISS
    determination of liver left/right asymmetry GO:0071910 IMP
    metanephric mesenchyme development GO:0072075 IEP
    mesonephric tubule development GO:0072164 IEP
    mesonephric duct development GO:0072177 IEP
    metanephric smooth muscle tissue development GO:0072208 IEP
    metanephric cortex development GO:0072214 IEP
    metanephric ascending thin limb development GO:0072218 IEP
    metanephric cortical collecting duct development GO:0072219 IEP
    metanephric distal tubule development GO:0072235 IEP
    metanephric S-shaped body morphogenesis GO:0072284 IEP
    regulation of calcium ion import GO:0090279 IDA
    inorganic cation transmembrane transport GO:0098662 IMP
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IMP
Subcellular Localization
    polycystin complex GO:0002133 IPI
    polycystin complex GO:0002133 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 IMP
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    cell-cell junction GO:0005911 ISS
    cilium GO:0005929 IDA
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    basal plasma membrane GO:0009925 IDA
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IEA
    lamellipodium GO:0030027 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    motile cilium GO:0031514 ISS
    cation channel complex GO:0034703 IDA
    ciliary basal body GO:0036064 IDA
    basal cortex GO:0045180 IDA
    ciliary membrane GO:0060170 IEA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    mitotic spindle GO:0072686 IDA
    non-motile cilium GO:0097730 ISS
    lumenal side of endoplasmic reticulum membrane GO:0098553 IDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 IDA
    migrasome GO:0140494 IDA
 Experiment description of studies that identified PKD2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PKD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C15orf32  
Affinity Capture-MS Homo sapiens
2 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
3 CANX 821
Co-fractionation Homo sapiens
4 IFITM5  
Affinity Capture-MS Homo sapiens
5 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FAM3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MAGEA8  
Two-hybrid Homo sapiens
8 OR10H1  
Affinity Capture-MS Homo sapiens
9 CYSRT1 375791
Two-hybrid Homo sapiens
10 GLRA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CLGN 1047
Affinity Capture-MS Homo sapiens
12 ALG5 29880
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RPN2 6185
Proximity Label-MS Homo sapiens
14 GRIN3B  
Affinity Capture-MS Homo sapiens
15 EXOC3 11336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C1D  
Affinity Capture-MS Homo sapiens
17 FOXP1 27086
Co-localization Homo sapiens
18 C15orf59  
Affinity Capture-MS Homo sapiens
19 CD300E  
Affinity Capture-MS Homo sapiens
20 CYP2J2  
Affinity Capture-MS Homo sapiens
21 CEP170 9859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TMEM248 55069
Affinity Capture-MS Homo sapiens
23 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NUMB 8650
Affinity Capture-MS Homo sapiens
25 Nrip3  
Affinity Capture-MS Mus musculus
26 TMPRSS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
28 CENPH  
Affinity Capture-MS Homo sapiens
29 SRPR 6734
Affinity Capture-MS Homo sapiens
30 MDFI  
Two-hybrid Homo sapiens
31 PKD2L2 27039
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PLSCR1 5359
Two-hybrid Homo sapiens
33 COG7 91949
Affinity Capture-MS Homo sapiens
34 S1PR1 1901
Affinity Capture-MS Homo sapiens
35 PKD1 5310
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
36 KRTAP10-3  
Two-hybrid Homo sapiens
37 C5AR1  
Affinity Capture-MS Homo sapiens
38 BCAS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TMEM169  
Affinity Capture-MS Homo sapiens
40 GOLGA2 2801
Co-fractionation Homo sapiens
41 ART4  
Affinity Capture-MS Homo sapiens
42 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GCC2 9648
Affinity Capture-MS Homo sapiens
45 TRPC1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
46 Chek1  
Affinity Capture-MS Mus musculus
47 LSMEM2  
Affinity Capture-MS Homo sapiens
48 SPCS1 28972
Affinity Capture-MS Homo sapiens
49 TPTE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CNGA4  
Affinity Capture-MS Homo sapiens
51 FBXO2 26232
Affinity Capture-MS Homo sapiens
52 OR6T1  
Affinity Capture-MS Homo sapiens
53 KRTAP10-8  
Two-hybrid Homo sapiens
54 ATP1A1 476
Co-fractionation Homo sapiens
55 KRTAP6-2  
Two-hybrid Homo sapiens
56 CCDC107  
Affinity Capture-MS Homo sapiens
57 TMC4  
Affinity Capture-MS Homo sapiens
58 CNGA3  
Affinity Capture-MS Homo sapiens
59 PKD2 5311
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
60 KRT40  
Two-hybrid Homo sapiens
61 Kctd5  
Affinity Capture-MS Mus musculus
62 TNNI3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 NTSR1  
Affinity Capture-MS Homo sapiens
64 HSF2BP  
Two-hybrid Homo sapiens
65 PIGH  
Affinity Capture-MS Homo sapiens
66 SEC62 7095
Proximity Label-MS Homo sapiens
67 LHFPL5  
Affinity Capture-MS Homo sapiens
68 DACT3  
Affinity Capture-MS Homo sapiens
69 RNF133  
Affinity Capture-MS Homo sapiens
70 C3orf52  
Affinity Capture-MS Homo sapiens
71 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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