Gene description for CPSF2
Gene name cleavage and polyadenylation specific factor 2, 100kDa
Gene symbol CPSF2
Other names/aliases CPSF100
Species Homo sapiens
 Database cross references - CPSF2
ExoCarta ExoCarta_53981
Vesiclepedia VP_53981
Entrez Gene 53981
HGNC 2325
MIM 606028
UniProt Q9P2I0  
 CPSF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CPSF2
Molecular Function
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
Biological Process
    mRNA 3'-end processing by stem-loop binding and cleavage GO:0006398 IBA
    mRNA 3'-end processing by stem-loop binding and cleavage GO:0006398 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IBA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
    membrane GO:0016020 HDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified CPSF2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CPSF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Proximity Label-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 MED17  
Co-fractionation Homo sapiens
4 PCF11 51585
Co-fractionation Homo sapiens
5 PSMB4 5692
Co-fractionation Homo sapiens
6 CSNK1A1 1452
Co-fractionation Homo sapiens
7 P4HA2 8974
Affinity Capture-MS Homo sapiens
8 KIF20A 10112
Affinity Capture-MS Homo sapiens
9 SAP30 8819
Co-fractionation Homo sapiens
10 UNKL  
Two-hybrid Homo sapiens
11 PRPF40A 55660
Affinity Capture-MS Homo sapiens
12 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ISY1 57461
Co-fractionation Homo sapiens
14 CPSF4  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
15 NPM1 4869
Affinity Capture-Western Homo sapiens
16 MDC1  
Affinity Capture-MS Homo sapiens
17 SNRPC 6631
Affinity Capture-MS Homo sapiens
18 Tagap  
Affinity Capture-MS Mus musculus
19 OBSL1 23363
Affinity Capture-MS Homo sapiens
20 ATG16L1 55054
Affinity Capture-MS Homo sapiens
21 PSMB6 5694
Co-fractionation Homo sapiens
22 MECP2 4204
Affinity Capture-MS Homo sapiens
23 MYCN  
Affinity Capture-MS Homo sapiens
24 PSMB3 5691
Co-fractionation Homo sapiens
25 RBM14 10432
Affinity Capture-MS Homo sapiens
26 KIF14 9928
Affinity Capture-MS Homo sapiens
27 CLK2 1196
Affinity Capture-MS Homo sapiens
28 SYMPK 8189
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
30 ECT2 1894
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 SUPT5H 6829
Co-fractionation Homo sapiens
33 CSTF2 1478
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
34 SRRT 51593
Affinity Capture-MS Homo sapiens
35 SMARCA4 6597
Co-fractionation Homo sapiens
36 BARD1 580
Affinity Capture-MS Homo sapiens
37 NTRK1 4914
Affinity Capture-MS Homo sapiens
38 ARHGAP11A  
Affinity Capture-MS Homo sapiens
39 RAD18  
Affinity Capture-MS Homo sapiens
40 NSRP1  
Affinity Capture-MS Homo sapiens
41 ARRDC3 57561
Affinity Capture-MS Homo sapiens
42 MBD3 53615
Co-fractionation Homo sapiens
43 CCNT1  
Affinity Capture-MS Homo sapiens
44 GATA3  
Proximity Label-MS Homo sapiens
45 RBBP6 5930
Affinity Capture-Western Homo sapiens
46 FAM76B  
Affinity Capture-MS Homo sapiens
47 GTF3C3 9330
Co-fractionation Homo sapiens
48 PSMA3 5684
Affinity Capture-MS Homo sapiens
49 CPSF1 29894
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CDC73  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 SMARCAD1  
Affinity Capture-MS Homo sapiens
52 CSTF2T  
Affinity Capture-Western Homo sapiens
53 SMARCA5 8467
Co-fractionation Homo sapiens
54 MRPS11  
Affinity Capture-MS Homo sapiens
55 WDR33 55339
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
58 FUS 2521
Affinity Capture-MS Homo sapiens
59 POLR2A 5430
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
60 TBP  
Affinity Capture-RNA Homo sapiens
61 GTF3C6  
Co-fractionation Homo sapiens
62 MYC  
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 FOXL1  
Affinity Capture-MS Homo sapiens
65 PLEKHG7  
Affinity Capture-MS Homo sapiens
66 TOP1 7150
Affinity Capture-MS Homo sapiens
67 FIP1L1 81608
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
68 PSMB5 5693
Co-fractionation Homo sapiens
69 PAPOLA 10914
Co-fractionation Homo sapiens
70 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
71 MTA2 9219
Co-fractionation Homo sapiens
72 RALBP1 10928
Co-fractionation Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 PARP1 142
Proximity Label-MS Homo sapiens
75 CEP170P1  
Affinity Capture-MS Homo sapiens
76 CPSF6 11052
Affinity Capture-MS Homo sapiens
77 SSU72 29101
Affinity Capture-MS Homo sapiens
78 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
79 DUSP3 1845
Affinity Capture-MS Homo sapiens
80 Pspc1  
Two-hybrid Mus musculus
81 MEN1 4221
Affinity Capture-MS Homo sapiens
82 POLE3  
Co-fractionation Homo sapiens
83 SNRPA 6626
Affinity Capture-MS Homo sapiens
84 SP7  
Proximity Label-MS Homo sapiens
85 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
86 UFL1 23376
Affinity Capture-MS Homo sapiens
87 KDM1A 23028
Co-fractionation Homo sapiens
88 NCL 4691
Co-fractionation Homo sapiens
89 EZH2  
Affinity Capture-MS Homo sapiens
90 CAD 790
Co-fractionation Homo sapiens
91 SNW1 22938
Affinity Capture-MS Homo sapiens
92 KRAS 3845
Negative Genetic Homo sapiens
93 C9orf72  
Affinity Capture-MS Homo sapiens
94 SMARCD3  
Co-fractionation Homo sapiens
95 CLP1  
Affinity Capture-Western Homo sapiens
96 HECW2  
Affinity Capture-MS Homo sapiens
97 CPSF3 51692
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
98 ASB7  
Affinity Capture-MS Homo sapiens
99 CDC5L 988
Affinity Capture-MS Homo sapiens
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