Gene description for RYR2
Gene name ryanodine receptor 2 (cardiac)
Gene symbol RYR2
Other names/aliases ARVC2
ARVD2
RYR-2
RyR
VTSIP
Species Homo sapiens
 Database cross references - RYR2
ExoCarta ExoCarta_6262
Vesiclepedia VP_6262
Entrez Gene 6262
HGNC 10484
MIM 180902
UniProt Q92736  
 RYR2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for RYR2
Molecular Function
    ryanodine-sensitive calcium-release channel activity GO:0005219 IBA
    ryanodine-sensitive calcium-release channel activity GO:0005219 IDA
    calcium channel activity GO:0005262 ISS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    calmodulin binding GO:0005516 IMP
    calmodulin binding GO:0005516 IPI
    calmodulin binding GO:0005516 ISS
    intracellularly gated calcium channel activity GO:0015278 IDA
    intracellularly gated calcium channel activity GO:0015278 ISS
    enzyme binding GO:0019899 IPI
    protein kinase A catalytic subunit binding GO:0034236 IDA
    protein kinase A regulatory subunit binding GO:0034237 IDA
    identical protein binding GO:0042802 IPI
    suramin binding GO:0043924 IMP
    calcium-induced calcium release activity GO:0048763 IDA
Biological Process
    response to hypoxia GO:0001666 ISS
    regulation of heart rate GO:0002027 IMP
    embryonic heart tube morphogenesis GO:0003143 ISS
    left ventricular cardiac muscle tissue morphogenesis GO:0003220 ISS
    cardiac muscle hypertrophy GO:0003300 ISS
    detection of calcium ion GO:0005513 IDA
    calcium ion transport GO:0006816 IDA
    intracellular calcium ion homeostasis GO:0006874 ISS
    striated muscle contraction GO:0006941 IBA
    positive regulation of heart rate GO:0010460 ISS
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 IC
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 ISS
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IMP
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IBA
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IDA
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IMP
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 ISS
    response to muscle activity GO:0014850 IMP
    calcium-mediated signaling GO:0019722 IDA
    calcium-mediated signaling GO:0019722 ISS
    response to caffeine GO:0031000 IDA
    response to muscle stretch GO:0035994 IMP
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    positive regulation of sequestering of calcium ion GO:0051284 IDA
    regulation of cytosolic calcium ion concentration GO:0051480 ISS
    establishment of localization in cell GO:0051649 IEA
    response to redox state GO:0051775 IDA
    regulation of cardiac muscle contraction GO:0055117 IMP
    cardiac muscle contraction GO:0060048 IMP
    calcium ion transport into cytosol GO:0060402 IDA
    sarcoplasmic reticulum calcium ion transport GO:0070296 TAS
    cellular response to caffeine GO:0071313 IDA
    cellular response to caffeine GO:0071313 ISS
    cellular response to epinephrine stimulus GO:0071872 ISS
    cellular response to epinephrine stimulus GO:0071872 TAS
    establishment of protein localization to endoplasmic reticulum GO:0072599 IDA
    ventricular cardiac muscle cell action potential GO:0086005 ISS
    Purkinje myocyte to ventricular cardiac muscle cell signaling GO:0086029 ISS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 IC
    type B pancreatic cell apoptotic process GO:0097050 IMP
    positive regulation of the force of heart contraction GO:0098735 IMP
    regulation of AV node cell action potential GO:0098904 IMP
    regulation of SA node cell action potential GO:0098907 IMP
    regulation of atrial cardiac muscle cell action potential GO:0098910 IMP
    regulation of ventricular cardiac muscle cell action potential GO:0098911 IMP
Subcellular Localization
    smooth endoplasmic reticulum GO:0005790 IBA
    plasma membrane GO:0005886 ISS
    junctional sarcoplasmic reticulum membrane GO:0014701 TAS
    membrane GO:0016020 IDA
    sarcoplasmic reticulum GO:0016529 IDA
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    Z disc GO:0030018 ISS
    protein-containing complex GO:0032991 IDA
    sarcoplasmic reticulum membrane GO:0033017 IBA
    sarcoplasmic reticulum membrane GO:0033017 ISS
    sarcoplasmic reticulum membrane GO:0033017 ISS
    sarcoplasmic reticulum membrane GO:0033017 TAS
    calcium channel complex GO:0034704 IBA
    calcium channel complex GO:0034704 IDA
    sarcolemma GO:0042383 IBA
 Experiment description of studies that identified RYR2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RYR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD5 4090
Two-hybrid Homo sapiens
2 PPP1CB 5500
Co-fractionation Homo sapiens
3 TERF2  
Affinity Capture-MS Homo sapiens
4 HSPB2  
Two-hybrid Homo sapiens
5 PSEN1 5663
Reconstituted Complex Homo sapiens
6 JPH3 57338
FRET Homo sapiens
7 JPH4 84502
FRET Homo sapiens
8 LINC00839  
Protein-RNA Homo sapiens
9 RYR2 6262
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
10 CACNA1C  
Reconstituted Complex Homo sapiens
11 RPL13A 23521
Cross-Linking-MS (XL-MS) Homo sapiens
12 PPP1CA 5499
Co-fractionation Homo sapiens
13 ALB 213
Affinity Capture-MS Homo sapiens
14 PRKACG  
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
15 NR3C1 2908
Proximity Label-MS Homo sapiens
16 NUP43 348995
Proximity Label-MS Homo sapiens
17 RYR3 6263
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 SNTA1 6640
Affinity Capture-MS Homo sapiens
19 PRKAR2B 5577
Co-fractionation Homo sapiens
20 PPP1CC 5501
Co-fractionation Homo sapiens
21 RYR1 6261
Affinity Capture-Western Homo sapiens
22 PRKACA 5566
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
23 TERF1 7013
Affinity Capture-MS Homo sapiens
24 AKAP6  
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
25 NCL 4691
Cross-Linking-MS (XL-MS) Homo sapiens
26 MDM2  
Reconstituted Complex Homo sapiens
27 GRIN2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 CACNA1D  
FRET Homo sapiens
29 KCNN4 3783
FRET Homo sapiens
30 PGAM1 5223
Cross-Linking-MS (XL-MS) Homo sapiens
31 FKBP1B  
Reconstituted Complex Homo sapiens
32 HOMER1  
Reconstituted Complex Homo sapiens
33 PRKAR2A 5576
Co-fractionation Homo sapiens
34 PRKACB 5567
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
35 SRI 6717
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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