Gene description for SLC7A1
Gene name solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Gene symbol SLC7A1
Other names/aliases ATRC1
CAT-1
ERR
HCAT1
REC1L
Species Homo sapiens
 Database cross references - SLC7A1
ExoCarta ExoCarta_6541
Vesiclepedia VP_6541
Entrez Gene 6541
HGNC 11057
MIM 104615
UniProt P30825  
 SLC7A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC7A1
Molecular Function
    L-ornithine transmembrane transporter activity GO:0000064 IBA
    L-ornithine transmembrane transporter activity GO:0000064 ISS
    virus receptor activity GO:0001618 IEA
    L-histidine transmembrane transporter activity GO:0005290 IDA
    protein binding GO:0005515 IPI
    amino acid transmembrane transporter activity GO:0015171 IDA
    amino acid transmembrane transporter activity GO:0015171 TAS
    basic amino acid transmembrane transporter activity GO:0015174 IDA
    basic amino acid transmembrane transporter activity GO:0015174 IMP
    L-lysine transmembrane transporter activity GO:0015189 IBA
    L-lysine transmembrane transporter activity GO:0015189 IDA
    L-arginine transmembrane transporter activity GO:0061459 IBA
    L-arginine transmembrane transporter activity GO:0061459 IDA
    L-arginine transmembrane transporter activity GO:0061459 IMP
    L-arginine transmembrane transporter activity GO:0061459 ISS
Biological Process
    amino acid transport GO:0006865 TAS
    L-amino acid transport GO:0015807 IDA
    lysine transport GO:0015819 IDA
    ornithine transport GO:0015822 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    symbiont entry into host cell GO:0046718 IEA
    amino acid import across plasma membrane GO:0089718 IDA
    L-arginine import across plasma membrane GO:0097638 IBA
    L-arginine import across plasma membrane GO:0097638 ISS
    transport across blood-brain barrier GO:0150104 NAS
    L-ornithine transmembrane transport GO:1903352 IBA
    L-ornithine transmembrane transport GO:1903352 ISS
    L-lysine transmembrane transport GO:1903401 IEA
    L-histidine import across plasma membrane GO:1903810 IDA
    L-arginine transmembrane transport GO:1903826 IDA
    L-arginine transmembrane transport GO:1903826 IMP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 ISS
    membrane GO:0016020 HDA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified SLC7A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
12
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
21
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
22
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
28
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC7A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C8A 731
Two-hybrid Homo sapiens
2 ZDHHC9  
Affinity Capture-MS Homo sapiens
3 TMBIM6 7009
Two-hybrid Homo sapiens
4 METTL7A 25840
Affinity Capture-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 SYNGR1 9145
Affinity Capture-MS Homo sapiens
7 LGALS9C  
Affinity Capture-MS Homo sapiens
8 ORMDL3 94103
Two-hybrid Homo sapiens
9 RPA2 6118
Proximity Label-MS Homo sapiens
10 GLB1 2720
Two-hybrid Homo sapiens
11 TMEM184A  
Affinity Capture-MS Homo sapiens
12 SLC2A1 6513
Affinity Capture-MS Homo sapiens
13 PTPN9 5780
Two-hybrid Homo sapiens
14 MAVS 57506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TMEM186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LAMP2 3920
Proximity Label-MS Homo sapiens
17 MFSD8 256471
Affinity Capture-MS Homo sapiens
18 CD79A  
Two-hybrid Homo sapiens
19 STX4 6810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 MGAT5 4249
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SLC18A2  
Two-hybrid Homo sapiens
23 B3GAT1  
Proximity Label-MS Homo sapiens
24 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
25 CMTM4  
Affinity Capture-MS Homo sapiens
26 CYB5R1 51706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SLC10A6  
Two-hybrid Homo sapiens
28 GPR55  
Affinity Capture-MS Homo sapiens
29 C10orf35  
Two-hybrid Homo sapiens
30 ADGRB3 577
Two-hybrid Homo sapiens
31 ZDHHC21  
Affinity Capture-MS Homo sapiens
32 MS4A13  
Two-hybrid Homo sapiens
33 PARL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SACM1L 22908
Two-hybrid Homo sapiens
35 SEC23A 10484
Two-hybrid Homo sapiens
36 RAB35 11021
Proximity Label-MS Homo sapiens
37 CA14 23632
Affinity Capture-MS Homo sapiens
38 GPRC5C 55890
Affinity Capture-MS Homo sapiens
39 GNPAT 8443
Affinity Capture-MS Homo sapiens
40 AGPAT4 56895
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MARCKS 4082
Proximity Label-MS Homo sapiens
42 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 DGAT1 8694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 LAMTOR1 55004
Proximity Label-MS Homo sapiens
45 KIAA1715 80856
Affinity Capture-MS Homo sapiens
46 CD70 970
Affinity Capture-MS Homo sapiens
47 RNF144A  
Two-hybrid Homo sapiens
48 MTCH2 23788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HERPUD2  
Two-hybrid Homo sapiens
50 HS2ST1 9653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CCL4L2  
Two-hybrid Homo sapiens
52 LEPROT 54741
Two-hybrid Homo sapiens
53 PDCD1LG2 80380
Two-hybrid Homo sapiens
54 RAB4A 5867
Proximity Label-MS Homo sapiens
55 ACP2 53
Affinity Capture-MS Homo sapiens
56 SLC39A2  
Two-hybrid Homo sapiens
57 UBXN8  
Affinity Capture-MS Homo sapiens
58 GPR152  
Two-hybrid Homo sapiens
59 PMAIP1  
Affinity Capture-MS Homo sapiens
60 DIRAS3  
Proximity Label-MS Homo sapiens
61 CCL4  
Two-hybrid Homo sapiens
62 APOA2 336
Two-hybrid Homo sapiens
63 TUSC5  
Two-hybrid Homo sapiens
64 LEMD2 221496
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 GPR182  
Affinity Capture-MS Homo sapiens
66 CREB3L1  
Two-hybrid Homo sapiens
67 SLC22A18 5002
Affinity Capture-MS Homo sapiens
68 RPN1 6184
Proximity Label-MS Homo sapiens
69 ARL13B 200894
Affinity Capture-MS Homo sapiens
70 VSTM1  
Affinity Capture-MS Homo sapiens
71 SCN3B  
Two-hybrid Homo sapiens
72 ELOVL5 60481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MFF 56947
Two-hybrid Homo sapiens
74 LGALS9 3965
Affinity Capture-MS Homo sapiens
75 SLC33A1 9197
Affinity Capture-MS Homo sapiens
76 ST6GAL2  
Two-hybrid Homo sapiens
77 TSPO2  
Two-hybrid Homo sapiens
78 CDIPT 10423
Two-hybrid Homo sapiens
79 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
80 CD40 958
Two-hybrid Homo sapiens
81 BCAP31 10134
Proximity Label-MS Homo sapiens
82 SLC30A2  
Two-hybrid Homo sapiens
83 MBOAT7 79143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 YIPF6 286451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 TVP23C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 MGARP  
Affinity Capture-MS Homo sapiens
88 HSD3B7 80270
Proximity Label-MS Homo sapiens
89 FITM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 STIM1 6786
Two-hybrid Homo sapiens
91 EGFR 1956
Negative Genetic Homo sapiens
92 ARF6 382
Proximity Label-MS Homo sapiens
93 TMEM11 8834
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 SLC39A1 27173
Affinity Capture-MS Homo sapiens
96 CMTM2  
Two-hybrid Homo sapiens
97 SGPP1 81537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CD27  
Affinity Capture-MS Homo sapiens
99 MAPK6  
Affinity Capture-MS Homo sapiens
100 TMEM237 65062
Two-hybrid Homo sapiens
101 RPA3 6119
Proximity Label-MS Homo sapiens
102 WRB 7485
Two-hybrid Homo sapiens
103 GIF  
Two-hybrid Homo sapiens
104 GPR45  
Affinity Capture-MS Homo sapiens
105 ACKR2  
Affinity Capture-MS Homo sapiens
106 YIPF4 84272
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
107 CERS6  
Affinity Capture-MS Homo sapiens
108 GALNT15  
Two-hybrid Homo sapiens
109 RHOB 388
Proximity Label-MS Homo sapiens
110 KRAS 3845
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
111 NAT14 57106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 FATE1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
113 C9orf91 203197
Affinity Capture-MS Homo sapiens
114 PTAFR  
Affinity Capture-MS Homo sapiens
115 LEPROTL1 23484
Two-hybrid Homo sapiens
116 FLVCR1 28982
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 EMC6 83460
Two-hybrid Homo sapiens
118 TMEM19  
Two-hybrid Homo sapiens
119 SLC19A2 10560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 PSCA 8000
Two-hybrid Homo sapiens
121 PIGW  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 FASN 2194
Positive Genetic Homo sapiens
123 GPR89A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 SYNGR2 9144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 PRAF2 11230
Affinity Capture-MS Homo sapiens
126 TP53 7157
Affinity Capture-MS Homo sapiens
127 MFSD4  
Affinity Capture-MS Homo sapiens
128 TMEM120B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 SLC35B2 347734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 FKRP 79147
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 TSPAN15 23555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 PNLIPRP1  
Two-hybrid Homo sapiens
133 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
134 AGPAT3 56894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 ATP6V0C 527
Two-hybrid Homo sapiens
136 BCL2L13 23786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 RAB2A 5862
Proximity Label-MS Homo sapiens
138 SEC62 7095
Proximity Label-MS Homo sapiens
139 NDC1 55706
Affinity Capture-MS Homo sapiens
140 SPPL2B 56928
Affinity Capture-MS Homo sapiens
141 C19orf52  
Affinity Capture-MS Homo sapiens
142 SMCO4  
Two-hybrid Homo sapiens
143 RAC3 5881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 TPST2 8459
Affinity Capture-MS Homo sapiens
145 VIPR1  
Affinity Capture-MS Homo sapiens
146 RAB5C 5878
Proximity Label-MS Homo sapiens
147 GOLM1 51280
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 CKAP4 10970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 RMDN3 55177
Affinity Capture-MS Homo sapiens
150 ALG11  
Affinity Capture-MS Homo sapiens
151 SLC52A1  
Two-hybrid Homo sapiens
152 ELOVL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 FCER1G 2207
Two-hybrid Homo sapiens
154 MGST3 4259
Two-hybrid Homo sapiens
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