Gene description for Rpa1
Gene name replication protein A1
Gene symbol Rpa1
Other names/aliases 5031405K23Rik
70kDa
AA589576
AW557552
RF-A
RP-A
Rpa
Species Mus musculus
 Database cross references - Rpa1
ExoCarta ExoCarta_68275
Vesiclepedia VP_68275
Entrez Gene 68275
UniProt Q8VEE4  
 Rpa1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpa1
Molecular Function
    chromatin binding GO:0003682 IDA
    damaged DNA binding GO:0003684 IBA
    damaged DNA binding GO:0003684 ISO
    damaged DNA binding GO:0003684 ISS
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISS
    single-stranded telomeric DNA binding GO:0043047 IBA
    metal ion binding GO:0046872 IEA
    G-rich strand telomeric DNA binding GO:0098505 ISO
    chromatin-protein adaptor activity GO:0140463 ISO
Biological Process
    telomere maintenance GO:0000723 ISO
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 IMP
    double-strand break repair via homologous recombination GO:0000724 ISO
    in utero embryonic development GO:0001701 IMP
    DNA replication GO:0006260 ISO
    DNA replication GO:0006260 ISS
    DNA unwinding involved in DNA replication GO:0006268 IBA
    DNA repair GO:0006281 ISO
    base-excision repair GO:0006284 ISO
    base-excision repair GO:0006284 ISS
    nucleotide-excision repair GO:0006289 IBA
    nucleotide-excision repair GO:0006289 ISO
    nucleotide-excision repair GO:0006289 ISS
    mismatch repair GO:0006298 ISO
    mismatch repair GO:0006298 ISS
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISS
    telomere maintenance via telomerase GO:0007004 IBA
    positive regulation of cell population proliferation GO:0008284 IMP
    hemopoiesis GO:0030097 IMP
    protein localization to chromosome GO:0034502 ISO
    protein localization to chromosome GO:0034502 ISS
    homeostasis of number of cells within a tissue GO:0048873 IMP
    chromosome organization GO:0051276 IMP
    meiotic cell cycle GO:0051321 IBA
    meiotic cell cycle GO:0051321 IDA
    protein localization to site of double-strand break GO:1990166 ISO
Subcellular Localization
    condensed chromosome GO:0000793 IDA
    condensed nuclear chromosome GO:0000794 IDA
    lateral element GO:0000800 IDA
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    DNA replication factor A complex GO:0005662 IBA
    DNA replication factor A complex GO:0005662 ISO
    DNA replication factor A complex GO:0005662 ISS
    PML body GO:0016605 ISO
    PML body GO:0016605 ISO
    site of double-strand break GO:0035861 ISO
    site of DNA damage GO:0090734 ISO
    site of DNA damage GO:0090734 ISS
 Experiment description of studies that identified Rpa1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zfml  
Co-fractionation Mus musculus
2 Raver1  
Co-fractionation Mus musculus
3 Rab5c 19345
Co-fractionation Mus musculus
4 Mycbp2 105689
Affinity Capture-MS Mus musculus
5 Rpa2  
Co-fractionation Mus musculus
6 Sec22b  
Co-fractionation Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
8 Cdc5l 71702
Co-fractionation Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Foxp3  
Affinity Capture-MS Mus musculus
11 Rab7 19349
Co-fractionation Mus musculus
12 Tfe3  
Affinity Capture-MS Mus musculus
13 Timeless  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 Pus1  
Co-fractionation Mus musculus
15 Csnk1a1 93687
Co-fractionation Mus musculus
16 Ntpcr  
Co-fractionation Mus musculus
17 Tmem173  
Proximity Label-MS Mus musculus
18 Atg12  
Positive Genetic Mus musculus
19 Zfr  
Co-fractionation Mus musculus
20 Myof 226101
Co-fractionation Mus musculus
21 Tfcp2l1  
Affinity Capture-MS Mus musculus
22 TCF3  
Reconstituted Complex Homo sapiens
23 Zbtb7b  
Affinity Capture-MS Mus musculus
24 Rab11b 19326
Co-fractionation Mus musculus
25 Atg16l1  
Affinity Capture-MS Mus musculus
26 Tipin  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
27 Kcna3  
Affinity Capture-MS Mus musculus
28 Rpa3  
Co-fractionation Mus musculus
29 Scamp3 24045
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Rpa1 is involved
PathwayEvidenceSource
Activation of ATR in response to replication stress IEA Reactome
Activation of the pre-replicative complex IEA Reactome
Base Excision Repair IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Chromosome Maintenance IEA Reactome
DNA Damage Bypass IEA Reactome
DNA Double-Strand Break Repair IEA Reactome
DNA Repair IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
DNA strand elongation IEA Reactome
Dual Incision in GG-NER IEA Reactome
Dual incision in TC-NER IEA Reactome
Extension of Telomeres IEA Reactome
Fanconi Anemia Pathway IEA Reactome
Formation of Incision Complex in GG-NER IEA Reactome
G1/S Transition IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Global Genome Nucleotide Excision Repair (GG-NER) IEA Reactome
HDR through Homologous Recombination (HRR) IEA Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) IEA Reactome
HDR through Single Strand Annealing (SSA) IEA Reactome
Homology Directed Repair IEA Reactome
Lagging Strand Synthesis IEA Reactome
Metabolism of proteins IEA Reactome
Mismatch Repair IEA Reactome
Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) IEA Reactome
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Nucleotide Excision Repair IEA Reactome
PCNA-Dependent Long Patch Base Excision Repair IEA Reactome
Post-translational protein modification IEA Reactome
Processing of DNA double-strand break ends IEA Reactome
Processive synthesis on the C-strand of the telomere IEA Reactome
Processive synthesis on the lagging strand IEA Reactome
Recognition of DNA damage by PCNA-containing replication complex IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Activity through Phosphorylation IEA Reactome
Removal of the Flap Intermediate IEA Reactome
Removal of the Flap Intermediate from the C-strand IEA Reactome
Resolution of Abasic Sites (AP sites) IEA Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of DNA damage response and repair proteins IEA Reactome
Synthesis of DNA IEA Reactome
Telomere C-strand (Lagging Strand) Synthesis IEA Reactome
Telomere Maintenance IEA Reactome
Termination of translesion DNA synthesis IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
Translesion Synthesis by POLH IEA Reactome
Translesion synthesis by POLI IEA Reactome
Translesion synthesis by POLK IEA Reactome
Translesion synthesis by REV1 IEA Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template IEA Reactome





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