Gene description for SYT5
Gene name synaptotagmin V
Gene symbol SYT5
Other names/aliases -
Species Homo sapiens
 Database cross references - SYT5
ExoCarta ExoCarta_6861
Vesiclepedia VP_6861
Entrez Gene 6861
HGNC 11513
MIM 600782
UniProt O00445  
 SYT5 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for SYT5
Molecular Function
    SNARE binding GO:0000149 IBA
    phosphatidylserine binding GO:0001786 IEA
    calcium ion binding GO:0005509 IDA
    calcium-dependent phospholipid binding GO:0005544 IBA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    syntaxin binding GO:0019905 IEA
    clathrin binding GO:0030276 IEA
    protein heterodimerization activity GO:0046982 IEA
    calcium ion sensor activity GO:0061891 IBA
Biological Process
    chemical synaptic transmission GO:0007268 TAS
    vesicle-mediated transport GO:0016192 IBA
    regulation of calcium ion-dependent exocytosis GO:0017158 IBA
    calcium-dependent activation of synaptic vesicle fusion GO:0099502 IBA
    regulation of synaptic vesicle exocytosis GO:2000300 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    axon GO:0030424 IBA
    synaptic vesicle membrane GO:0030672 IBA
    dense core granule GO:0031045 IBA
    neuronal cell body GO:0043025 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    recycling endosome membrane GO:0055038 IEA
    exocytic vesicle GO:0070382 IBA
    neuronal dense core vesicle membrane GO:0099012 IEA
    proximal neuron projection GO:1990769 IEA
 Experiment description of studies that identified SYT5 in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SYT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HTR6  
Two-hybrid Homo sapiens
2 SYT5 6861
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 POLR2E 5434
Two-hybrid Homo sapiens
4 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PALM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SYT2  
Affinity Capture-MS Homo sapiens
7 TP53 7157
Negative Genetic Homo sapiens
8 NEU2  
Affinity Capture-MS Homo sapiens
9 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SYT5 is involved
PathwayEvidenceSource
Integration of energy metabolism IEA Reactome
Metabolism IEA Reactome
Regulation of insulin secretion IEA Reactome





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