Gene description for Cul5
Gene name cullin 5
Gene symbol Cul5
Other names/aliases 4921514I20Rik
8430423K24Rik
AI852817
C030032G03Rik
C330021I08Rik
VACM-1
VACM1
Species Mus musculus
 Database cross references - Cul5
ExoCarta ExoCarta_75717
Vesiclepedia VP_75717
Entrez Gene 75717
UniProt Q9D5V5  
 Cul5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cul5
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IBA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin ligase complex scaffold activity GO:0160072 IMP
Biological Process
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 IGI
    cerebral cortex radially oriented cell migration GO:0021799 IMP
    radial glia guided migration of Purkinje cell GO:0021942 IMP
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    reelin-mediated signaling pathway GO:0038026 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    regulation of cytosolic calcium ion concentration GO:0051480 ISO
    regulation of neuron migration GO:2001222 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IBA
    Cul5-RING ubiquitin ligase complex GO:0031466 IBA
    Cul5-RING ubiquitin ligase complex GO:0031466 IMP
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
    site of DNA damage GO:0090734 ISO
    site of DNA damage GO:0090734 ISS
 Experiment description of studies that identified Cul5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cul5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf7  
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 SOCS3 9021
Affinity Capture-Western Homo sapiens
4 Ube2f  
Co-fractionation Mus musculus
5 Socs4  
Reconstituted Complex Mus musculus
6 Socs7  
Reconstituted Complex Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Dcun1d5  
Co-fractionation Mus musculus
9 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
10 Socs3  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
11 Socs5  
Reconstituted Complex Mus musculus
12 Socs6  
Reconstituted Complex Mus musculus
13 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
14 Upf1 19704
Co-fractionation Mus musculus
15 Traf6  
Affinity Capture-Western Mus musculus
16 Sae1 56459
Co-fractionation Mus musculus
17 SOCS4  
Affinity Capture-MS Homo sapiens
18 Cish  
Reconstituted Complex Mus musculus
19 Krt77 406220
Co-fractionation Mus musculus
20 Socs2  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
21 Nrbp1 192292
Affinity Capture-Western Mus musculus
22 Bcar1  
Affinity Capture-Western Mus musculus
23 Dab1  
Affinity Capture-Western Mus musculus
24 Luzp1  
Co-fractionation Mus musculus
25 Fancd2  
Affinity Capture-MS Mus musculus
26 Socs1  
Reconstituted Complex Mus musculus
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