Gene description for Arf6
Gene name ADP-ribosylation factor 6
Gene symbol Arf6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arf6
ExoCarta ExoCarta_79121
Vesiclepedia VP_79121
Entrez Gene 79121
UniProt P62332  
 Arf6 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Arf6
Molecular Function
    GTPase activity GO:0003924 IEA
    G protein activity GO:0003925 ISO
    G protein activity GO:0003925 ISS
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    thioesterase binding GO:0031996 ISO
    signaling adaptor activity GO:0035591 ISO
Biological Process
    liver development GO:0001889 ISO
    intracellular protein transport GO:0006886 IBA
    nervous system development GO:0007399 IEA
    regulation of neuron projection development GO:0010975 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    vesicle-mediated transport GO:0016192 IBA
    actin cytoskeleton organization GO:0030036 TAS
    cell differentiation GO:0030154 IEA
    positive regulation of actin filament polymerization GO:0030838 ISO
    cortical actin cytoskeleton organization GO:0030866 ISO
    endocytic recycling GO:0032456 IDA
    endocytic recycling GO:0032456 ISO
    protein localization to cell surface GO:0034394 ISO
    regulation of Rac protein signal transduction GO:0035020 ISO
    protein localization to endosome GO:0036010 IBA
    protein localization to endosome GO:0036010 ISO
    protein localization to endosome GO:0036010 ISS
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    positive regulation of protein secretion GO:0050714 ISO
    regulation of filopodium assembly GO:0051489 ISO
    positive regulation of keratinocyte migration GO:0051549 ISO
    regulation of dendritic spine development GO:0060998 IBA
    regulation of dendritic spine development GO:0060998 IMP
    regulation of dendritic spine development GO:0060998 ISO
    protein localization to plasma membrane GO:0072659 ISO
    establishment of epithelial cell polarity GO:0090162 ISO
    ruffle assembly GO:0097178 ISO
    hepatocyte apoptotic process GO:0097284 ISO
    maintenance of postsynaptic density structure GO:0099562 IDA
    maintenance of postsynaptic density structure GO:0099562 IMP
    positive regulation of focal adhesion disassembly GO:0120183 ISO
    erythrocyte apoptotic process GO:1902217 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of mitotic cytokinetic process GO:1903438 ISO
    protein localization to cleavage furrow GO:1905345 ISO
    regulation of presynapse assembly GO:1905606 IDA
    regulation of presynapse assembly GO:1905606 IMP
    cellular response to nerve growth factor stimulus GO:1990090 IDA
    negative regulation of protein localization to cell surface GO:2000009 ISO
    negative regulation of dendrite development GO:2000171 IMP
Subcellular Localization
    ruffle GO:0001726 IBA
    ruffle GO:0001726 IDA
    ruffle GO:0001726 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 IDA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 ISO
    membrane GO:0016020 ISO
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    midbody GO:0030496 ISO
    filopodium membrane GO:0031527 ISO
    early endosome membrane GO:0031901 IEA
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISO
    cleavage furrow GO:0032154 ISS
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IBA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    exocytic vesicle GO:0070382 ISO
    Flemming body GO:0090543 ISO
    Flemming body GO:0090543 ISS
    presynapse GO:0098793 IDA
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Arf6 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Arf6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Abcd3  
Co-fractionation Rattus norvegicus
2 Pex3  
Co-fractionation Rattus norvegicus
3 Tfrc 64678
Reconstituted Complex Rattus norvegicus
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