Gene description for CALU
Gene name calumenin
Gene symbol CALU
Other names/aliases -
Species Homo sapiens
 Database cross references - CALU
ExoCarta ExoCarta_813
Vesiclepedia VP_813
Entrez Gene 813
HGNC 1458
MIM 603420
UniProt O43852  
 CALU identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
 Gene ontology annotations for CALU
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
Subcellular Localization
    extracellular region GO:0005576 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 HDA
    sarcoplasmic reticulum lumen GO:0033018 IEA
    melanosome GO:0042470 IEA
 Experiment description of studies that identified CALU in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
22
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 136
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
27
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for CALU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 UGGT2 55757
Proximity Label-MS Homo sapiens
3 ACTR2 10097
Co-fractionation Homo sapiens
4 ITGB1 3688
Proximity Label-MS Homo sapiens
5 KLF8  
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 SARS2 54938
Co-fractionation Homo sapiens
8 PTPN22  
Affinity Capture-MS Homo sapiens
9 ARHGAP39  
Affinity Capture-MS Homo sapiens
10 ERO1L 30001
Proximity Label-MS Homo sapiens
11 ARPC2 10109
Co-fractionation Homo sapiens
12 SUCO  
Affinity Capture-MS Homo sapiens
13 SH3GL1 6455
Co-fractionation Homo sapiens
14 BTF3 689
Affinity Capture-MS Homo sapiens
15 DBN1 1627
Co-fractionation Homo sapiens
16 NOP56 10528
Proximity Label-MS Homo sapiens
17 HSPA13 6782
Proximity Label-MS Homo sapiens
18 PRPH 5630
Co-fractionation Homo sapiens
19 TRIM33 51592
Affinity Capture-MS Homo sapiens
20 CDC25B 994
Affinity Capture-MS Homo sapiens
21 CSNK2A1 1457
Biochemical Activity Homo sapiens
22 NENF 29937
Proximity Label-MS Homo sapiens
23 HSPA4L 22824
Co-fractionation Homo sapiens
24 LRRC59 55379
Proximity Label-MS Homo sapiens
25 ASPH 444
Proximity Label-MS Homo sapiens
26 TMEM256 254863
Co-fractionation Homo sapiens
27 PLOD1 5351
Proximity Label-MS Homo sapiens
28 ERO1LB 56605
Proximity Label-MS Homo sapiens
29 KIAA1429 25962
Affinity Capture-MS Homo sapiens
30 LAMC1 3915
Proximity Label-MS Homo sapiens
31 TKT 7086
Co-fractionation Homo sapiens
32 QSOX2 169714
Proximity Label-MS Homo sapiens
33 TP53RK 112858
Co-fractionation Homo sapiens
34 RDX 5962
Co-fractionation Homo sapiens
35 BRD4 23476
Affinity Capture-MS Homo sapiens
36 PRKCDBP 112464
Co-fractionation Homo sapiens
37 KIF23 9493
Affinity Capture-MS Homo sapiens
38 MCM2 4171
Affinity Capture-MS Homo sapiens
39 RAF1 5894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 H1FX 8971
Co-fractionation Homo sapiens
41 ACTR3 10096
Co-fractionation Homo sapiens
42 RCN2 5955
Proximity Label-MS Homo sapiens
43 ARPC4 10093
Co-fractionation Homo sapiens
44 FBXW7  
Affinity Capture-MS Homo sapiens
45 TOR1AIP1 26092
Proximity Label-MS Homo sapiens
46 TMED2 10959
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
47 NUP210 23225
Proximity Label-MS Homo sapiens
48 WIZ 58525
Co-fractionation Homo sapiens
49 OBSL1 23363
Affinity Capture-MS Homo sapiens
50 PPEF1  
Affinity Capture-MS Homo sapiens
51 DDOST 1650
Proximity Label-MS Homo sapiens
52 UBQLN1 29979
Two-hybrid Homo sapiens
53 Cenpe  
Affinity Capture-MS Mus musculus
54 IGF2 3481
Affinity Capture-MS Homo sapiens
55 IGFL3  
Affinity Capture-MS Homo sapiens
56 CCDC47 57003
Proximity Label-MS Homo sapiens
57 P3H1 64175
Proximity Label-MS Homo sapiens
58 ATG16L1 55054
Affinity Capture-MS Homo sapiens
59 TOMM40 10452
Co-fractionation Homo sapiens
60 GTSE1 51512
Affinity Capture-MS Homo sapiens
61 CALR 811
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
62 ARPC1B 10095
Co-fractionation Homo sapiens
63 SNX27 81609
Affinity Capture-MS Homo sapiens
64 ARPC5L 81873
Co-fractionation Homo sapiens
65 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
66 FAF2 23197
Co-fractionation Homo sapiens
67 SMARCA2 6595
Affinity Capture-MS Homo sapiens
68 CLGN 1047
Co-fractionation Homo sapiens
69 ARHGEF15  
Affinity Capture-MS Homo sapiens
70 FAM20C 56975
Affinity Capture-MS Homo sapiens
71 NACA2 342538
Co-fractionation Homo sapiens
72 TMTC3 160418
Proximity Label-MS Homo sapiens
73 EMC1 23065
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
74 LMAN1 3998
Proximity Label-MS Homo sapiens
75 CHID1 66005
Proximity Label-MS Homo sapiens
76 PXMP2  
Proximity Label-MS Homo sapiens
77 CHMP4C 92421
Affinity Capture-MS Homo sapiens
78 AURKA 6790
Affinity Capture-MS Homo sapiens
79 Mgp 17313
Affinity Capture-MS Mus musculus
80 NSUN2 54888
Co-fractionation Homo sapiens
81 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
82 DPP8 54878
Co-fractionation Homo sapiens
83 IPO11 51194
Co-fractionation Homo sapiens
84 P4HA1 5033
Proximity Label-MS Homo sapiens
85 PLOD3 8985
Proximity Label-MS Homo sapiens
86 TPD52L2 7165
Co-fractionation Homo sapiens
87 IKBIP 121457
Proximity Label-MS Homo sapiens
88 IRAK1  
Affinity Capture-MS Homo sapiens
89 ABCE1 6059
Cross-Linking-MS (XL-MS) Homo sapiens
90 TMX1 81542
Proximity Label-MS Homo sapiens
91 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 SAE1 10055
Co-fractionation Homo sapiens
93 TMEM165 55858
Co-fractionation Homo sapiens
94 HSP90B1 7184
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
95 PPT2  
Co-fractionation Homo sapiens
96 UBE2I 7329
Biochemical Activity Homo sapiens
97 BAG6 7917
Affinity Capture-MS Homo sapiens
98 ATF2  
Affinity Capture-MS Homo sapiens
99 TPD52 7163
Co-fractionation Homo sapiens
100 Vav2  
Affinity Capture-MS Mus musculus
101 Myo9b  
Affinity Capture-MS Rattus norvegicus
102 PDLIM5 10611
Co-fractionation Homo sapiens
103 WDR70 55100
Co-fractionation Homo sapiens
104 CLCC1 23155
Proximity Label-MS Homo sapiens
105 SP1  
Affinity Capture-MS Homo sapiens
106 VCP 7415
Co-fractionation Homo sapiens
107 NTRK1 4914
Affinity Capture-MS Homo sapiens
108 STT3B 201595
Proximity Label-MS Homo sapiens
109 SAR1B 51128
Affinity Capture-MS Homo sapiens
110 KDELC2 143888
Proximity Label-MS Homo sapiens
111 PDIA6 10130
Proximity Label-MS Homo sapiens
112 DNAJB9 4189
Proximity Label-MS Homo sapiens
113 IQCB1  
Affinity Capture-MS Homo sapiens
114 MIA3 375056
Proximity Label-MS Homo sapiens
115 NOS2  
Affinity Capture-MS Homo sapiens
116 P4HB 5034
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
117 THG1L 54974
Co-fractionation Homo sapiens
118 RPN1 6184
Proximity Label-MS Homo sapiens
119 Mad2l2  
Affinity Capture-MS Mus musculus
120 CEP76  
Affinity Capture-MS Homo sapiens
121 DNAJC30  
Proximity Label-MS Homo sapiens
122 PRKAR1B  
Co-fractionation Homo sapiens
123 SDF4 51150
Proximity Label-MS Homo sapiens
124 SDF2L1 23753
Proximity Label-MS Homo sapiens
125 VDAC2 7417
Co-fractionation Homo sapiens
126 CEBPA  
Protein-peptide Homo sapiens
127 SEC61B 10952
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
128 DNAJB11 51726
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
129 EMID1  
Affinity Capture-MS Homo sapiens
130 CTNNAL1 8727
Two-hybrid Homo sapiens
131 MESDC2 23184
Proximity Label-MS Homo sapiens
132 MTR 4548
Co-fractionation Homo sapiens
133 NUCB2 4925
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
134 SEC63 11231
Proximity Label-MS Homo sapiens
135 TAF7  
Co-fractionation Homo sapiens
136 HSPA5 3309
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
137 RPL32 6161
Co-fractionation Homo sapiens
138 Trim28 21849
Affinity Capture-MS Mus musculus
139 HADHB 3032
Co-fractionation Homo sapiens
140 PPP4R2  
Cross-Linking-MS (XL-MS) Homo sapiens
141 CANX 821
Proximity Label-MS Homo sapiens
142 TRIM31  
Affinity Capture-MS Homo sapiens
143 DNAJB12 54788
Proximity Label-MS Homo sapiens
144 BPTF 2186
Co-fractionation Homo sapiens
145 CALR3  
Proximity Label-MS Homo sapiens
146 GANAB 23193
Proximity Label-MS Homo sapiens
147 FKBP1A 2280
Cross-Linking-MS (XL-MS) Homo sapiens
148 CHST8 64377
Affinity Capture-MS Homo sapiens
149 UBQLN2 29978
Co-fractionation Homo sapiens
150 MRPS7 51081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 ASPM 259266
Affinity Capture-MS Homo sapiens
152 GOLT1B 51026
Affinity Capture-MS Homo sapiens
153 SUMF2 25870
Proximity Label-MS Homo sapiens
154 BCAP31 10134
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
155 CUL3 8452
Affinity Capture-MS Homo sapiens
156 NR3C1 2908
Proximity Label-MS Homo sapiens
157 RPL21 6144
Co-fractionation Homo sapiens
158 PDIA3 2923
Proximity Label-MS Homo sapiens
159 EGFR 1956
Co-fractionation Homo sapiens
160 OSGEP 55644
Co-fractionation Homo sapiens
161 HSD17B10 3028
Co-fractionation Homo sapiens
162 PLOD2 5352
Proximity Label-MS Homo sapiens
163 GAA 2548
Co-fractionation Homo sapiens
164 FBXO6 26270
Affinity Capture-MS Homo sapiens
165 CNPY3 10695
Proximity Label-MS Homo sapiens
166 KIF4B  
Proximity Label-MS Homo sapiens
167 ERP29 10961
Proximity Label-MS Homo sapiens
168 RCN1 5954
Proximity Label-MS Homo sapiens
169 SEL1L 6400
Proximity Label-MS Homo sapiens
170 APCS 325
Reconstituted Complex Homo sapiens
171 SSH3 54961
Affinity Capture-MS Homo sapiens
172 DUS3L 56931
Co-fractionation Homo sapiens
173 AURKB 9212
Affinity Capture-MS Homo sapiens
174 UGGT1 56886
Proximity Label-MS Homo sapiens
175 MYCN  
Affinity Capture-MS Homo sapiens
176 CEP250 11190
Affinity Capture-MS Homo sapiens
177 UBA2 10054
Co-fractionation Homo sapiens
178 UMPS 7372
Two-hybrid Homo sapiens
179 KIF1BP 26128
Co-fractionation Homo sapiens
180 MYC  
Affinity Capture-MS Homo sapiens
181 EIF2D 1939
Co-fractionation Homo sapiens
182 Mis12  
Affinity Capture-MS Mus musculus
183 PLIN3 10226
Co-fractionation Homo sapiens
184 NCLN 56926
Proximity Label-MS Homo sapiens
185 PTPRN  
Proximity Label-MS Homo sapiens
186 DNAJC10 54431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
187 SH3GLB2 56904
Co-fractionation Homo sapiens
188 DNAJC3 5611
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
189 HYOU1 10525
Proximity Label-MS Homo sapiens
190 ILK 3611
Affinity Capture-MS Homo sapiens
191 XRCC3  
Affinity Capture-MS Homo sapiens
192 FKBP10 60681
Proximity Label-MS Homo sapiens
193 MSN 4478
Co-fractionation Homo sapiens
194 AARS 16
Co-fractionation Homo sapiens
195 MTM1 4534
Affinity Capture-MS Homo sapiens
196 ARAF 369
Affinity Capture-MS Homo sapiens
197 EEF2K  
Co-fractionation Homo sapiens
198 BRD3 8019
Affinity Capture-MS Homo sapiens
199 AKAP1 8165
Proximity Label-MS Homo sapiens
200 NIF3L1 60491
Co-fractionation Homo sapiens
201 HUWE1 10075
Affinity Capture-MS Homo sapiens
202 ARPC1A 10552
Co-fractionation Homo sapiens
203 ARPC3 10094
Co-fractionation Homo sapiens
204 LAMB1 3912
Proximity Label-MS Homo sapiens
205 PRKCSH 5589
Proximity Label-MS Homo sapiens
206 TMED10 10972
Proximity Label-MS Homo sapiens
207 RPP30 10556
Co-fractionation Homo sapiens
208 CKAP4 10970
Proximity Label-MS Homo sapiens
209 MRPS34 65993
Cross-Linking-MS (XL-MS) Homo sapiens
210 TRMT10A 93587
Co-fractionation Homo sapiens
211 ICT1 3396
Co-fractionation Homo sapiens
212 PDIA4 9601
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
213 KDELC1 79070
Proximity Label-MS Homo sapiens
214 MLEC 9761
Proximity Label-MS Homo sapiens
215 TXNDC5 81567
Proximity Label-MS Homo sapiens
216 CNPY2 10330
Proximity Label-MS Homo sapiens
217 PRKACA 5566
Co-fractionation Homo sapiens
218 PTBP3 9991
Co-fractionation Homo sapiens
219 PTPN2 5771
Affinity Capture-MS Homo sapiens
220 PRMT5 10419
Two-hybrid Homo sapiens
221 NUCB1 4924
Proximity Label-MS Homo sapiens
222 DDX58 23586
Affinity Capture-RNA Homo sapiens
223 TPRKB 51002
Co-fractionation Homo sapiens
224 AARSD1 80755
Co-fractionation Homo sapiens
225 PCNA 5111
Co-fractionation Homo sapiens
226 ZNF217 7764
Affinity Capture-MS Homo sapiens
227 LRPAP1 4043
Proximity Label-MS Homo sapiens
228 HIC2 23119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
229 ZPBP  
Two-hybrid Homo sapiens
230 SH3GLB1 51100
Co-fractionation Homo sapiens
231 HSF1  
Co-fractionation Homo sapiens
232 HARS 3035
Co-fractionation Homo sapiens
233 MAGT1 84061
Proximity Label-MS Homo sapiens
234 RPRD1B 58490
Co-fractionation Homo sapiens
235 BRD1 23774
Affinity Capture-MS Homo sapiens
236 SERBP1 26135
Affinity Capture-MS Homo sapiens
237 PTGES3 10728
Co-fractionation Homo sapiens
238 CLPTM1L 81037
Proximity Label-MS Homo sapiens
239 B3GNT2 10678
Affinity Capture-MS Homo sapiens
240 COLGALT1 79709
Proximity Label-MS Homo sapiens
241 VDAC3 7419
Co-fractionation Homo sapiens
242 MAST3  
Affinity Capture-MS Homo sapiens
243 WARS 7453
Co-fractionation Homo sapiens
244 RPA2 6118
Co-fractionation Homo sapiens
245 LIMK2 3985
Affinity Capture-MS Homo sapiens
246 BRI3BP 140707
Proximity Label-MS Homo sapiens
247 RANGAP1 5905
Co-fractionation Homo sapiens
248 IARS2 55699
Co-fractionation Homo sapiens
249 EP300 2033
Affinity Capture-MS Homo sapiens
250 PPIB 5479
Proximity Label-MS Homo sapiens
251 CNTROB  
Affinity Capture-MS Homo sapiens
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