Gene description for LIMK2
Gene name LIM domain kinase 2
Gene symbol LIMK2
Other names/aliases -
Species Homo sapiens
 Database cross references - LIMK2
ExoCarta ExoCarta_3985
Vesiclepedia VP_3985
Entrez Gene 3985
HGNC 6614
MIM 601988
UniProt P53671  
 LIMK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LIMK2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IMP
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    spermatogenesis GO:0007283 IEA
    phosphorylation GO:0016310 TAS
    actin cytoskeleton organization GO:0030036 IBA
    astral microtubule organization GO:0030953 IDA
    establishment of vesicle localization GO:0051650 IMP
    head development GO:0060322 IEA
    cornea development in camera-type eye GO:0061303 IEA
    positive regulation of protein localization to nucleus GO:1900182 IMP
    negative regulation of cilium assembly GO:1902018 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 TAS
    cis-Golgi network GO:0005801 IEA
    centrosome GO:0005813 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified LIMK2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIMK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC25A13 10165
Affinity Capture-MS Homo sapiens
2 LIMK1 3984
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Negative Genetic Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 TTC1 7265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ROCK1 6093
Biochemical Activity Homo sapiens
7 HSPA8 3312
Affinity Capture-MS Homo sapiens
8 BAG2 9532
Affinity Capture-MS Homo sapiens
9 RUVBL2 10856
Affinity Capture-MS Homo sapiens
10 DNAJA2 10294
Affinity Capture-MS Homo sapiens
11 CALU 813
Affinity Capture-MS Homo sapiens
12 SLC25A1 6576
Affinity Capture-MS Homo sapiens
13 EEF2 1938
Affinity Capture-MS Homo sapiens
14 CDC37 11140
Affinity Capture-MS Homo sapiens
15 HIPK3 10114
Two-hybrid Homo sapiens
16 LMNA 4000
Affinity Capture-MS Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 EMD 2010
Affinity Capture-MS Homo sapiens
19 HSPB1 3315
Affinity Capture-MS Homo sapiens
20 SLC25A22  
Affinity Capture-MS Homo sapiens
21 SFTPA2  
Affinity Capture-MS Homo sapiens
22 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
23 CFL1 1072
Affinity Capture-Western Homo sapiens
24 HSP90B1 7184
Affinity Capture-MS Homo sapiens
25 RCN1 5954
Affinity Capture-MS Homo sapiens
26 TUBA4A 7277
Affinity Capture-MS Homo sapiens
27 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FKBP5 2289
Affinity Capture-MS Homo sapiens
29 USMG5 84833
Affinity Capture-MS Homo sapiens
30 MYC  
Dosage Lethality Homo sapiens
31 CDK4 1019
Affinity Capture-MS Homo sapiens
32 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CECR2  
Affinity Capture-MS Homo sapiens
34 ATP1A1 476
Affinity Capture-MS Homo sapiens
35 TRIM35 23087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 GPR182  
Affinity Capture-MS Homo sapiens
37 RCN2 5955
Affinity Capture-MS Homo sapiens
38 DNAJA1 3301
Affinity Capture-MS Homo sapiens
39 HIST1H1A 3024
Biochemical Activity Homo sapiens
40 UNC119 9094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SLC31A1 1317
Affinity Capture-MS Homo sapiens
42 PPP1R14B 26472
Affinity Capture-MS Homo sapiens
43 LIMK2 3985
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
44 NOTCH1 4851
Affinity Capture-MS Homo sapiens
45 MBP 4155
Biochemical Activity Homo sapiens
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