Gene description for CD247
Gene name CD247 molecule
Gene symbol CD247
Other names/aliases CD3-ZETA
CD3H
CD3Q
CD3Z
IMD25
T3Z
TCRZ
Species Homo sapiens
 Database cross references - CD247
ExoCarta ExoCarta_919
Vesiclepedia VP_919
Entrez Gene 919
HGNC 1677
MIM 186780
UniProt P20963  
 CD247 identified in sEVs derived from the following tissue/cell type
Bone marrow cells 15908444    
T cells 11907077    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for CD247
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IC
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein heterodimerization activity GO:0046982 IDA
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    adaptive immune response GO:0002250 NAS
    cell surface receptor signaling pathway GO:0007166 IC
    Fc-gamma receptor signaling pathway GO:0038094 IDA
    gamma-delta T cell activation GO:0046629 NAS
    alpha-beta T cell activation GO:0046631 NAS
    T cell receptor signaling pathway GO:0050852 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    T cell receptor signaling pathway GO:0050852 NAS
    protein complex oligomerization GO:0051259 EXP
    protein complex oligomerization GO:0051259 IPI
    protein-containing complex assembly GO:0065003 IDA
    positive regulation of protein localization to cell surface GO:2000010 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    Fc-gamma receptor III complex GO:0033001 IDA
    T cell receptor complex GO:0042101 IDA
    alpha-beta T cell receptor complex GO:0042105 IBA
    alpha-beta T cell receptor complex GO:0042105 IDA
    alpha-beta T cell receptor complex GO:0042105 IPI
    alpha-beta T cell receptor complex GO:0042105 ISS
    gamma-delta T cell receptor complex GO:0042106 NAS
 Experiment description of studies that identified CD247 in sEVs
1
Experiment ID 42
MISEV standards
✔
EM
Biophysical techniques
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors "Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C"
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 17
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors "Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C"
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
E+ cells
MART-1+ T cell
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 18
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors "Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C"
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD247
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Affinity Capture-MS Homo sapiens
2 CD38 952
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
3 SLA2 84174
Affinity Capture-Western Homo sapiens
4 LAT 27040
Affinity Capture-Western Homo sapiens
5 ICK  
Affinity Capture-Western Homo sapiens
6 PTPRC 5788
Reconstituted Complex Homo sapiens
7 STAT5B 6777
Affinity Capture-Western Homo sapiens
8 PTPN22  
Affinity Capture-Western Homo sapiens
9 CSK 1445
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 PCGF2 7703
Two-hybrid Homo sapiens
11 STAT5A 6776
Affinity Capture-Western Homo sapiens
12 VTN 7448
Two-hybrid Homo sapiens
13 C1orf123 54987
Affinity Capture-MS Homo sapiens
14 RNF41  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 SLA  
Affinity Capture-Western Homo sapiens
16 LCK 3932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 TRAT1 50852
Affinity Capture-Western Homo sapiens
18 ZNF281  
Two-hybrid Homo sapiens
19 GAB2 9846
Affinity Capture-Western Homo sapiens
20 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 JAK3 3718
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 COL1A1 1277
Affinity Capture-MS Homo sapiens
23 TUBA1B 10376
Affinity Capture-Western Homo sapiens
24 UBASH3A 53347
Affinity Capture-Western Homo sapiens
25 NCR1  
Affinity Capture-Western Homo sapiens
26 ZAP70 7535
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 UNC119 9094
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 DOCK2 1794
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
29 PIK3R1 5295
Affinity Capture-Western Homo sapiens
30 SHC3  
Affinity Capture-Western Homo sapiens
31 CD3E 916
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
32 GOPC 57120
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CD247 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Costimulation by the CD28 family TAS Reactome
Disease TAS Reactome
Downstream TCR signaling TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR activation IEA Reactome
FCGR activation TAS Reactome
FCGR3A-mediated IL10 synthesis TAS Reactome
FCGR3A-mediated phagocytosis TAS Reactome
Generation of second messenger molecules TAS Reactome
HIV Infection TAS Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival TAS Reactome
Leishmania phagocytosis TAS Reactome
Nef and signal transduction TAS Reactome
Parasite infection TAS Reactome
Parasitic Infection Pathways TAS Reactome
PD-1 signaling TAS Reactome
Phosphorylation of CD3 and TCR zeta chains TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Role of phospholipids in phagocytosis TAS Reactome
TCR signaling TAS Reactome
The role of Nef in HIV-1 replication and disease pathogenesis TAS Reactome
Translocation of ZAP-70 to Immunological synapse TAS Reactome
Viral Infection Pathways TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here