Gene description for STAT5B
Gene name signal transducer and activator of transcription 5B
Gene symbol STAT5B
Other names/aliases STAT5
Species Homo sapiens
 Database cross references - STAT5B
ExoCarta ExoCarta_6777
Vesiclepedia VP_6777
Entrez Gene 6777
HGNC 11367
MIM 604260
UniProt P51692  
 STAT5B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for STAT5B
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    chromatin binding GO:0003682 ISS
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    nuclear glucocorticoid receptor binding GO:0035259 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein dimerization activity GO:0046983 ISS
Biological Process
    mitotic cell cycle GO:0000278 IEA
    luteinization GO:0001553 IEA
    natural killer cell differentiation GO:0001779 IEA
    natural killer cell proliferation GO:0001787 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 IEA
    defense response GO:0006952 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 NAS
    female pregnancy GO:0007565 IEA
    lactation GO:0007595 IEA
    regulation of steroid metabolic process GO:0019218 IEA
    cytokine-mediated signaling pathway GO:0019221 IBA
    taurine metabolic process GO:0019530 ISS
    lipid storage GO:0019915 IEA
    B cell differentiation GO:0030183 IEA
    erythrocyte differentiation GO:0030218 IEA
    regulation of epithelial cell differentiation GO:0030856 IEA
    response to estradiol GO:0032355 IDA
    positive regulation of interleukin-2 production GO:0032743 IEA
    positive regulation of natural killer cell proliferation GO:0032819 IEA
    positive regulation of natural killer cell differentiation GO:0032825 IEA
    cellular response to hormone stimulus GO:0032870 IDA
    myeloid cell apoptotic process GO:0033028 IEA
    negative regulation of myeloid cell apoptotic process GO:0033033 IEA
    T cell differentiation in thymus GO:0033077 IEA
    erythropoietin-mediated signaling pathway GO:0038162 IDA
    regulation of multicellular organism growth GO:0040014 ISS
    positive regulation of multicellular organism growth GO:0040018 IEA
    positive regulation of activated T cell proliferation GO:0042104 IEA
    regulation of cell population proliferation GO:0042127 IBA
    natural killer cell mediated cytotoxicity GO:0042267 IEA
    progesterone metabolic process GO:0042448 IEA
    gamma-delta T cell differentiation GO:0042492 IEA
    T cell homeostasis GO:0043029 IEA
    response to peptide hormone GO:0043434 IBA
    positive regulation of B cell differentiation GO:0045579 IEA
    positive regulation of gamma-delta T cell differentiation GO:0045588 IEA
    negative regulation of erythrocyte differentiation GO:0045647 IEA
    positive regulation of erythrocyte differentiation GO:0045648 IDA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IEA
    development of secondary female sexual characteristics GO:0046543 IEA
    development of secondary male sexual characteristics GO:0046544 IEA
    Peyer's patch development GO:0048541 IEA
    positive regulation of inflammatory response GO:0050729 IEA
    activated T cell proliferation GO:0050798 IEA
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IBA
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IDA
    response to interleukin-2 GO:0070669 IEA
    response to interleukin-4 GO:0070670 IEA
    response to interleukin-15 GO:0070672 IEA
    cellular response to growth factor stimulus GO:0071363 ISS
    cellular response to epidermal growth factor stimulus GO:0071364 ISS
    mast cell migration GO:0097531 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    RNA polymerase II transcription regulator complex GO:0090575 IBA
    RNA polymerase II transcription regulator complex GO:0090575 NAS
 Experiment description of studies that identified STAT5B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for STAT5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 MVP 9961
Affinity Capture-MS Homo sapiens
3 MCMBP 79892
Co-fractionation Homo sapiens
4 C18orf8 29919
Co-fractionation Homo sapiens
5 ALAD 210
Co-fractionation Homo sapiens
6 PTPN11 5781
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
7 SEC24A 10802
Co-fractionation Homo sapiens
8 DPP9 91039
Co-fractionation Homo sapiens
9 NIF3L1 60491
Co-fractionation Homo sapiens
10 SHC1 6464
Affinity Capture-Western Homo sapiens
11 STAT5B 6777
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 TFG 10342
Two-hybrid Homo sapiens
13 FLOT1 10211
Affinity Capture-MS Homo sapiens
14 NMI  
Two-hybrid Homo sapiens
15 PTPN6 5777
Affinity Capture-Western Homo sapiens
16 STAT5A 6776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ASF1B  
Affinity Capture-MS Homo sapiens
18 STAC  
Two-hybrid Homo sapiens
19 UBE2M 9040
Affinity Capture-MS Homo sapiens
20 CALM2 805
Affinity Capture-MS Homo sapiens
21 NACA 4666
Affinity Capture-MS Homo sapiens
22 DLD 1738
Affinity Capture-MS Homo sapiens
23 DBN1 1627
Affinity Capture-MS Homo sapiens
24 KIT 3815
Biochemical Activity Homo sapiens
25 PDHA1 5160
Affinity Capture-MS Homo sapiens
26 ADAM32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FLOT2 2319
Affinity Capture-MS Homo sapiens
28 NR3C1 2908
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 LMO4  
Two-hybrid Homo sapiens
30 SURF2  
Co-fractionation Homo sapiens
31 INSR 3643
Affinity Capture-Western Homo sapiens
32 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
33 PDHB 5162
Affinity Capture-MS Homo sapiens
34 PGR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 CDKN1A  
Two-hybrid Homo sapiens
36 PTPN1 5770
Protein-peptide Homo sapiens
37 KANSL3  
Affinity Capture-MS Homo sapiens
38 HIVEP1 3096
Affinity Capture-MS Homo sapiens
39 AFTPH  
Two-hybrid Homo sapiens
40 ELP2 55250
Reconstituted Complex Homo sapiens
41 APP 351
Reconstituted Complex Homo sapiens
42 MAPK1 5594
Co-fractionation Homo sapiens
43 POU2F1 5451
Affinity Capture-Western Homo sapiens
44 CD247 919
Affinity Capture-Western Homo sapiens
45 PUS7L  
Affinity Capture-MS Homo sapiens
46 JAK3 3718
Two-hybrid Homo sapiens
47 PSMC3 5702
Affinity Capture-MS Homo sapiens
48 FGFR2 2263
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 TPM1 7168
Affinity Capture-MS Homo sapiens
50 C9orf156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SNX3 8724
Affinity Capture-MS Homo sapiens
52 SERTAD1  
Two-hybrid Homo sapiens
53 IL15  
Two-hybrid Homo sapiens
54 TSSK3  
Two-hybrid Homo sapiens
55 JAK1 3716
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 CTLA4  
Co-localization Homo sapiens
57 CHAF1A  
Two-hybrid Homo sapiens
58 NTRK1 4914
Affinity Capture-MS Homo sapiens
59 CCR5 1234
Affinity Capture-Western Homo sapiens
60 MRPS6  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
61 PDHX 8050
Affinity Capture-MS Homo sapiens
62 HARS2 23438
Affinity Capture-MS Homo sapiens
63 PRLR  
Affinity Capture-Western Homo sapiens
64 RPRD1B 58490
Co-fractionation Homo sapiens
65 IMPDH2 3615
Affinity Capture-MS Homo sapiens
66 IL2RB  
Reconstituted Complex Homo sapiens
67 GHR  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
68 ANKMY2 57037
Co-fractionation Homo sapiens
69 STAT3 6774
Co-localization Homo sapiens
70 MED25  
Two-hybrid Homo sapiens
71 DMRTA1 63951
Two-hybrid Homo sapiens
72 HAX1  
Two-hybrid Homo sapiens
73 TPM3 7170
Affinity Capture-MS Homo sapiens
74 AARS 16
Co-fractionation Homo sapiens
75 DLAT 1737
Affinity Capture-MS Homo sapiens
76 JAK2 3717
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
77 RPA3 6119
Affinity Capture-MS Homo sapiens
78 TOM1L1 10040
Co-fractionation Homo sapiens
79 TPM4 7171
Affinity Capture-MS Homo sapiens
80 RPRD1A  
Co-fractionation Homo sapiens
81 RBBP4 5928
Two-hybrid Homo sapiens
82 HNRNPA2B1 3181
Two-hybrid Homo sapiens
83 PIK3R3 8503
Two-hybrid Homo sapiens
84 SUOX  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which STAT5B is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Cytokine Signaling in Immune system IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream signal transduction TAS Reactome
Erythropoietin activates STAT5 IEA Reactome
FGFR1 mutant receptor activation TAS Reactome
FLT3 Signaling IEA Reactome
FLT3 signaling in disease TAS Reactome
Growth hormone receptor signaling IEA Reactome
Growth hormone receptor signaling TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Inactivation of CSF3 (G-CSF) signaling IEA Reactome
Interleukin-15 signaling TAS Reactome
Interleukin-2 family signaling TAS Reactome
Interleukin-2 family signaling IEA Reactome
Interleukin-2 signaling IEA Reactome
Interleukin-2 signaling TAS Reactome
Interleukin-20 family signaling TAS Reactome
Interleukin-21 signaling TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Interleukin-7 signaling IEA Reactome
Interleukin-7 signaling TAS Reactome
Interleukin-9 signaling TAS Reactome
Prolactin receptor signaling IEA Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by cytosolic FGFR1 fusion mutants TAS Reactome
Signaling by Erythropoietin IEA Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FLT3 fusion proteins TAS Reactome
Signaling by FLT3 ITD and TKD mutants TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Interleukins IEA Reactome
Signaling by KIT in disease TAS Reactome
Signaling by Leptin IEA Reactome
Signaling by PDGF TAS Reactome
Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by SCF-KIT TAS Reactome
STAT5 Activation IEA Reactome
STAT5 activation downstream of FLT3 ITD mutants TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here