Gene description for AKAP7
Gene name A kinase (PRKA) anchor protein 7
Gene symbol AKAP7
Other names/aliases AKAP15
AKAP18
Species Homo sapiens
 Database cross references - AKAP7
ExoCarta ExoCarta_9465
Vesiclepedia VP_9465
Entrez Gene 9465
HGNC 377
MIM 604693
UniProt O43687   Q9P0M2  
 AKAP7 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
 Gene ontology annotations for AKAP7
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A binding GO:0051018 IDA
    protein kinase A binding GO:0051018 IPI
    protein kinase A binding GO:0051018 TAS
Biological Process
    action potential GO:0001508 IC
    monoatomic ion transport GO:0006811 TAS
    protein localization GO:0008104 IC
    regulation of protein kinase A signaling GO:0010738 IBA
    intracellular signal transduction GO:0035556 TAS
    modulation of chemical synaptic transmission GO:0050804 IEA
    regulation of membrane repolarization GO:0060306 IDA
    cellular response to cAMP GO:0071320 IDA
    positive regulation of potassium ion transmembrane transport GO:1901381 IDA
    positive regulation of delayed rectifier potassium channel activity GO:1902261 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IDA
    lateral plasma membrane GO:0016328 IDA
    protein-containing complex GO:0032991 IDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
 Experiment description of studies that identified AKAP7 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 622
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 623
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with IPMN with microinvasive cancer (IPMNca)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 625
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with benign pancreatic disease
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AKAP7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ROPN1  
Two-hybrid Homo sapiens
2 SEC63 11231
Affinity Capture-MS Homo sapiens
3 URM1  
Affinity Capture-MS Homo sapiens
4 PLS1 5357
Affinity Capture-MS Homo sapiens
5 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 USP4 7375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CTU1 90353
Affinity Capture-MS Homo sapiens
9 USP15 9958
Affinity Capture-MS Homo sapiens
10 SNRPB 6628
Affinity Capture-MS Homo sapiens
11 PRKAR2A 5576
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 PRKAR1A 5573
Two-hybrid Homo sapiens
13 ROPN1L  
Two-hybrid Homo sapiens
14 PRKACG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TAC3  
Affinity Capture-MS Homo sapiens
16 LSM4 25804
Affinity Capture-MS Homo sapiens
17 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SPA17  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
19 MEPCE 56257
Affinity Capture-MS Homo sapiens
20 LSM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PRKAR2B 5577
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TUBB2B 347733
Affinity Capture-MS Homo sapiens
24 PRKACA 5566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DCAF6 55827
Affinity Capture-MS Homo sapiens
26 LSM7  
Affinity Capture-MS Homo sapiens
27 C8orf34  
Two-hybrid Homo sapiens
28 LSM3 27258
Affinity Capture-MS Homo sapiens
29 ACVR2A  
Affinity Capture-MS Homo sapiens
30 DCAF10  
Affinity Capture-MS Homo sapiens
31 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LSM6 11157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PRPF4 9128
Affinity Capture-MS Homo sapiens
34 CEP76  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 PRKAR1B  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
36 ALG11  
Affinity Capture-MS Homo sapiens
37 FBXW5  
Affinity Capture-MS Homo sapiens
38 PRKACB 5567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SNRPD2 6633
Affinity Capture-MS Homo sapiens
40 SNRPD3 6634
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AKAP7 is involved
No pathways found





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