Gene description for CHKA
Gene name choline kinase alpha
Gene symbol CHKA
Other names/aliases CHK
CK
CKI
EK
Species Homo sapiens
 Database cross references - CHKA
ExoCarta ExoCarta_1119
Vesiclepedia VP_1119
Entrez Gene 1119
HGNC 1937
MIM 118491
UniProt P35790  
 CHKA identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CHKA
Molecular Function
    choline kinase activity GO:0004103 IBA
    choline kinase activity GO:0004103 IDA
    cholinesterase activity GO:0004104 IEA
    ethanolamine kinase activity GO:0004305 IBA
    ethanolamine kinase activity GO:0004305 IDA
    protein tyrosine kinase activity GO:0004713 IDA
    protein binding GO:0005515 IDA
    ATP binding GO:0005524 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    protein homodimerization activity GO:0042803 IDA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    lipid metabolic process GO:0006629 TAS
    phosphatidylethanolamine biosynthetic process GO:0006646 IBA
    phosphatidylethanolamine biosynthetic process GO:0006646 IDA
    phosphatidylethanolamine biosynthetic process GO:0006646 IEA
    phosphatidylcholine biosynthetic process GO:0006656 IDA
    CDP-choline pathway GO:0006657 IBA
    CDP-choline pathway GO:0006657 IEA
    lipid transport GO:0006869 TAS
    cellular response to glucose starvation GO:0042149 IDA
    lipid droplet disassembly GO:1905691 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    lipid droplet GO:0005811 IDA
    lipid droplet GO:0005811 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CHKA in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CHKA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Two-hybrid Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 IDE 3416
Affinity Capture-MS Homo sapiens
4 FASN 2194
Negative Genetic Homo sapiens
5 SKP2  
Biochemical Activity Homo sapiens
6 DEPTOR 64798
Biochemical Activity Homo sapiens
7 FCHSD2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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