Gene description for MAN1B1
Gene name mannosidase, alpha, class 1B, member 1
Gene symbol MAN1B1
Other names/aliases ERMAN1
MANA-ER
MRT15
Species Homo sapiens
 Database cross references - MAN1B1
ExoCarta ExoCarta_11253
Vesiclepedia VP_11253
Entrez Gene 11253
HGNC 6823
MIM 604346
UniProt Q9UKM7  
 MAN1B1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MAN1B1
Molecular Function
    mannosyl-oligosaccharide 1,2-alpha-mannosidase activity GO:0004571 IBA
    mannosyl-oligosaccharide 1,2-alpha-mannosidase activity GO:0004571 IDA
    mannosyl-oligosaccharide 1,2-alpha-mannosidase activity GO:0004571 IMP
    mannosyl-oligosaccharide 1,2-alpha-mannosidase activity GO:0004571 TAS
    calcium ion binding GO:0005509 TAS
Biological Process
    mannoprotein catabolic process GO:0006058 IDA
    mannoprotein catabolic process GO:0006058 IMP
    protein glycosylation GO:0006486 IEA
    oligosaccharide metabolic process GO:0009311 TAS
    viral protein processing GO:0019082 TAS
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
    protein alpha-1,2-demannosylation GO:0036508 NAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    membrane GO:0016020 TAS
    cytoplasmic vesicle GO:0031410 IDA
    endoplasmic reticulum quality control compartment GO:0044322 IDA
    endoplasmic reticulum quality control compartment GO:0044322 TAS
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified MAN1B1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAN1B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCNDBP1 23582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NAAA  
Affinity Capture-MS Homo sapiens
3 ICOSLG  
Affinity Capture-MS Homo sapiens
4 PCDHB7  
Affinity Capture-MS Homo sapiens
5 FGF14  
Affinity Capture-MS Homo sapiens
6 GP9 2815
Affinity Capture-MS Homo sapiens
7 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TNFSF8  
Affinity Capture-MS Homo sapiens
9 COMMD5 28991
Affinity Capture-MS Homo sapiens
10 TPTE  
Affinity Capture-MS Homo sapiens
11 FAM96A  
Affinity Capture-MS Homo sapiens
12 CUL5 8065
Affinity Capture-MS Homo sapiens
13 EDEM1  
Affinity Capture-MS Homo sapiens
14 MGARP  
Affinity Capture-MS Homo sapiens
15 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 TMEM9B 56674
Affinity Capture-MS Homo sapiens
17 GAA 2548
Affinity Capture-MS Homo sapiens
18 AQP3  
Affinity Capture-MS Homo sapiens
19 DDX58 23586
Affinity Capture-RNA Homo sapiens
20 ADAM7  
Affinity Capture-MS Homo sapiens
21 SPCS3 60559
Affinity Capture-MS Homo sapiens
22 VSIG4  
Affinity Capture-MS Homo sapiens
23 IL13 3596
Affinity Capture-MS Homo sapiens
24 KCNE3  
Affinity Capture-MS Homo sapiens
25 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
26 TEX264 51368
Affinity Capture-MS Homo sapiens
27 PLD3 23646
Affinity Capture-MS Homo sapiens
28 BRICD5  
Affinity Capture-MS Homo sapiens
29 Uso1 56041
Affinity Capture-MS Mus musculus
30 IL12RB1  
Affinity Capture-MS Homo sapiens
31 GPR45  
Affinity Capture-MS Homo sapiens
32 GPR182  
Affinity Capture-MS Homo sapiens
33 PML 5371
Affinity Capture-MS Homo sapiens
34 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
35 DNM2 1785
Two-hybrid Homo sapiens
36 PIGH  
Affinity Capture-MS Homo sapiens
37 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PTPRO 5800
Affinity Capture-MS Homo sapiens
39 GNAI3 2773
Affinity Capture-MS Homo sapiens
40 PCDHB3  
Affinity Capture-MS Homo sapiens
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