Gene description for DCTN3
Gene name dynactin 3 (p22)
Gene symbol DCTN3
Other names/aliases DCTN-22
DCTN22
Species Homo sapiens
 Database cross references - DCTN3
ExoCarta ExoCarta_11258
Vesiclepedia VP_11258
Entrez Gene 11258
HGNC 2713
MIM 607387
UniProt O75935  
 DCTN3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DCTN3
Molecular Function
    structural molecule activity GO:0005198 TAS
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    microtubule-based process GO:0007017 IEA
    cytoskeleton-dependent cytokinesis GO:0061640 IBA
    cytoskeleton-dependent cytokinesis GO:0061640 IDA
Subcellular Localization
    kinetochore GO:0000776 IEA
    nucleolus GO:0005730 IDA
    centrosome GO:0005813 IDA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    dynactin complex GO:0005869 IBA
    dynactin complex GO:0005869 IPI
    midbody GO:0030496 IEA
    cleavage furrow GO:0032154 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified DCTN3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DCTN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCF3 55324
Affinity Capture-MS Homo sapiens
2 CLIP1 6249
Affinity Capture-MS Homo sapiens
3 Dctn1 13191
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
4 DCTN6 10671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RPS19BP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DYNC1I2 1781
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SEPT14  
Affinity Capture-MS Homo sapiens
9 RPS6KA4 8986
Affinity Capture-MS Homo sapiens
10 DCTN2 10540
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
11 RIC8A 60626
Affinity Capture-MS Homo sapiens
12 TRIM55  
Two-hybrid Homo sapiens
13 ZNF398  
Affinity Capture-MS Homo sapiens
14 RIPK1 8737
Affinity Capture-MS Homo sapiens
15 DUSP28  
Affinity Capture-MS Homo sapiens
16 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 IRF2BP1 26145
Affinity Capture-MS Homo sapiens
18 DCTN4 51164
Proximity Label-MS Homo sapiens
19 ACTR1A 10121
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
20 MAD2L2 10459
Affinity Capture-MS Homo sapiens
21 DYNLL1 8655
Affinity Capture-MS Homo sapiens
22 MED22  
Affinity Capture-MS Homo sapiens
23 Dctn4  
Affinity Capture-MS Mus musculus
24 Dctn5  
Affinity Capture-MS Mus musculus
25 RPS6KA2  
Affinity Capture-MS Homo sapiens
26 GSK3B 2932
Two-hybrid Homo sapiens
27 N4BP2L1  
Affinity Capture-MS Homo sapiens
28 C15orf59  
Affinity Capture-MS Homo sapiens
29 C1orf106  
Affinity Capture-MS Homo sapiens
30 AURKA 6790
Affinity Capture-MS Homo sapiens
31 ACTA2 59
Affinity Capture-MS Homo sapiens
32 MAPK8IP3  
Affinity Capture-MS Homo sapiens
33 KRT34  
Two-hybrid Homo sapiens
34 SYMPK 8189
Affinity Capture-MS Homo sapiens
35 COASY 80347
Affinity Capture-MS Homo sapiens
36 MARCH7  
Affinity Capture-MS Homo sapiens
37 CAPZA1 829
Affinity Capture-MS Homo sapiens
38 DCTN5 84516
Affinity Capture-MS Homo sapiens
39 CCAR2 57805
Co-fractionation Homo sapiens
40 FHL2 2274
Co-fractionation Homo sapiens
41 MAPRE1 22919
Affinity Capture-MS Homo sapiens
42 TNKS1BP1 85456
Co-fractionation Homo sapiens
43 LRIF1  
Affinity Capture-MS Homo sapiens
44 PDXDC1 23042
Affinity Capture-MS Homo sapiens
45 FOXK2  
Affinity Capture-MS Homo sapiens
46 RIN1 9610
Affinity Capture-MS Homo sapiens
47 DCTN1 1639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FLNB 2317
Co-fractionation Homo sapiens
49 STIL  
Affinity Capture-MS Homo sapiens
50 MIS18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 AQP12B  
Affinity Capture-MS Homo sapiens
52 VCPIP1 80124
Affinity Capture-MS Homo sapiens
53 ACTR10 55860
Co-fractionation Homo sapiens
54 ACTG1 71
Affinity Capture-MS Homo sapiens
55 UBXN6 80700
Affinity Capture-MS Homo sapiens
56 FAM89B  
Affinity Capture-MS Homo sapiens
57 DYNLT3 6990
Co-fractionation Homo sapiens
58 SNAP29 9342
Affinity Capture-MS Homo sapiens
59 ATXN1 6310
Two-hybrid Homo sapiens
60 LEO1 123169
Co-fractionation Homo sapiens
61 LMNB1 4001
Co-fractionation Homo sapiens
62 NACC2  
Affinity Capture-MS Homo sapiens
63 UBE2L6 9246
Affinity Capture-MS Homo sapiens
64 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Negative Genetic Homo sapiens
66 THBS1 7057
Affinity Capture-MS Homo sapiens
67 THAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DUSP13  
Affinity Capture-MS Homo sapiens
69 Dctn2 69654
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
70 BIRC6 57448
Affinity Capture-MS Homo sapiens
71 CST4 1472
Affinity Capture-MS Homo sapiens
72 C4BPB 725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 NIN 51199
Proximity Label-MS Homo sapiens
74 RGPD5  
Affinity Capture-MS Homo sapiens
75 DIS3 22894
Affinity Capture-MS Homo sapiens
76 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
77 USP19 10869
Affinity Capture-MS Homo sapiens
78 DEFB127  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 USHBP1  
Two-hybrid Homo sapiens
80 L1TD1  
Affinity Capture-MS Homo sapiens
81 CBWD1 55871
Affinity Capture-MS Homo sapiens
82 CCL4L1  
Affinity Capture-MS Homo sapiens
83 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 RGL3  
Affinity Capture-MS Homo sapiens
85 RELA 5970
Affinity Capture-MS Homo sapiens
86 NUP54 53371
Two-hybrid Homo sapiens
87 CA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 RAD17  
Affinity Capture-MS Homo sapiens
90 THUMPD3 25917
Affinity Capture-MS Homo sapiens
91 NMI  
Affinity Capture-MS Homo sapiens
92 VPS37C 55048
Two-hybrid Homo sapiens
93 HSPA8 3312
Affinity Capture-MS Homo sapiens
94 Dync1li1 235661
Affinity Capture-MS Mus musculus
95 UQCRH 7388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 CHTF18 63922
Affinity Capture-MS Homo sapiens
97 Capza1 12340
Affinity Capture-MS Mus musculus
98 POMP  
Affinity Capture-MS Homo sapiens
99 CXCL14  
Affinity Capture-MS Homo sapiens
100 ACTR1B 10120
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 PHF19  
Affinity Capture-MS Homo sapiens
102 CSPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 SELO 83642
Affinity Capture-MS Homo sapiens
104 TRIP12 9320
Affinity Capture-MS Homo sapiens
105 CLASP1 23332
Affinity Capture-MS Homo sapiens
106 Actr1b 226977
Affinity Capture-MS Mus musculus
107 Dctn3  
Affinity Capture-MS Mus musculus
108 NEDD1 121441
Affinity Capture-MS Homo sapiens
109 ZG16B 124220
Affinity Capture-MS Homo sapiens
110 AKR7A2 8574
Affinity Capture-MS Homo sapiens
111 Actr1a 54130
Affinity Capture-MS Mus musculus
112 PAF1 54623
Affinity Capture-MS Homo sapiens
113 NUP85 79902
Co-fractionation Homo sapiens
114 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
116 EDRF1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DCTN3 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
Asparagine N-linked glycosylation TAS Reactome
AURKA Activation by TPX2 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Centrosome maturation TAS Reactome
Cilium Assembly TAS Reactome
COPI-independent Golgi-to-ER retrograde traffic TAS Reactome
COPI-mediated anterograde transport TAS Reactome
ER to Golgi Anterograde Transport TAS Reactome
G2/M Transition TAS Reactome
Golgi-to-ER retrograde transport TAS Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand TAS Reactome
Immune System TAS Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic TAS Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
Membrane Trafficking TAS Reactome
Metabolism of proteins TAS Reactome
MHC class II antigen presentation TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Prometaphase TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
Post-translational protein modification TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Transport to the Golgi and subsequent modification TAS Reactome
Vesicle-mediated transport TAS Reactome





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