Gene description for Adam10
Gene name a disintegrin and metallopeptidase domain 10
Gene symbol Adam10
Other names/aliases 1700031C13Rik
MADM
kuz
kuzbanian
Species Mus musculus
 Database cross references - Adam10
ExoCarta ExoCarta_11487
Vesiclepedia VP_11487
Entrez Gene 11487
UniProt O35598  
 Adam10 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Plasma 32132711    
Plasma 32132711    
Plasma 32132711    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adam10
Molecular Function
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 IMP
    endopeptidase activity GO:0004175 ISO
    metalloendopeptidase activity GO:0004222 ISO
    metalloendopeptidase activity GO:0004222 ISS
    metalloendopeptidase activity GO:0004222 TAS
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IDA
    metallopeptidase activity GO:0008237 IMP
    metallopeptidase activity GO:0008237 ISO
    SH3 domain binding GO:0017124 IEA
    protein kinase binding GO:0019901 IDA
    SH2 domain binding GO:0042169 NAS
    protein homodimerization activity GO:0042803 IPI
    metal ion binding GO:0046872 IEA
    molecular adaptor activity GO:0060090 ISO
    metallodipeptidase activity GO:0070573 IMP
    metalloendopeptidase activity involved in amyloid precursor protein catabolic process GO:1902945 IBA
    metalloendopeptidase activity involved in amyloid precursor protein catabolic process GO:1902945 IEA
    metalloendopeptidase activity involved in amyloid precursor protein catabolic process GO:1902945 ISO
Biological Process
    in utero embryonic development GO:0001701 IMP
    protein phosphorylation GO:0006468 IDA
    membrane protein ectodomain proteolysis GO:0006509 IBA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    membrane protein ectodomain proteolysis GO:0006509 IMP
    membrane protein ectodomain proteolysis GO:0006509 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISS
    nucleocytoplasmic transport GO:0006913 NAS
    negative regulation of cell adhesion GO:0007162 ISO
    negative regulation of cell adhesion GO:0007162 ISS
    Notch signaling pathway GO:0007219 IBA
    Notch signaling pathway GO:0007219 IMP
    Notch receptor processing GO:0007220 NAS
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    regulation of Notch signaling pathway GO:0008593 IMP
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of T cell chemotaxis GO:0010820 IEA
    positive regulation of T cell chemotaxis GO:0010820 ISO
    protein processing GO:0016485 IDA
    positive regulation of cell growth GO:0030307 IEA
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell migration GO:0030335 ISO
    amyloid-beta formation GO:0034205 ISO
    adherens junction organization GO:0034332 IMP
    response to tumor necrosis factor GO:0034612 IEA
    response to tumor necrosis factor GO:0034612 ISO
    epidermal growth factor receptor ligand maturation GO:0038004 IMP
    monocyte activation GO:0042117 IEA
    monocyte activation GO:0042117 ISO
    amyloid precursor protein catabolic process GO:0042987 IEA
    amyloid precursor protein catabolic process GO:0042987 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    pore complex assembly GO:0046931 IMP
    pore complex assembly GO:0046931 ISO
    constitutive protein ectodomain proteolysis GO:0051089 IEA
    constitutive protein ectodomain proteolysis GO:0051089 ISO
    regulation of dendritic spine morphogenesis GO:0061001 ISO
    cochlea development GO:0090102 IMP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 EXP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IDA
    postsynapse organization GO:0099173 EXP
    postsynapse organization GO:0099173 IDA
    regulation of postsynapse organization GO:0099175 EXP
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IEP
    regulation of postsynapse organization GO:0099175 IMP
    protein catabolic process at postsynapse GO:0140249 EXP
    protein catabolic process at postsynapse GO:0140249 IDA
    protein catabolic process at postsynapse GO:0140249 IEP
    protein catabolic process at postsynapse GO:0140249 IMP
    signaling receptor ligand precursor processing GO:0140448 IMP
    signaling receptor ligand precursor processing GO:0140448 ISO
    regulation of vasculature development GO:1901342 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    Golgi-associated vesicle GO:0005798 ISO
    Golgi-associated vesicle GO:0005798 ISS
    trans-Golgi network GO:0005802 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 IEA
    synaptic vesicle GO:0008021 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    postsynaptic density GO:0014069 IDA
    clathrin-coated vesicle GO:0030136 IEA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    postsynaptic membrane GO:0045211 ISO
    pore complex GO:0046930 IMP
    pore complex GO:0046930 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    perinuclear endoplasmic reticulum GO:0097038 IEA
    perinuclear endoplasmic reticulum GO:0097038 ISO
    synaptic membrane GO:0097060 IBA
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 ISO
    tetraspanin-enriched microdomain GO:0097197 IEA
    tetraspanin-enriched microdomain GO:0097197 ISO
    postsynapse GO:0098794 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Adam10 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 333
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 334
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 335
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adam10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Anxa6 11749
Co-fractionation Mus musculus
2 Dlg1 13383
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Igsf8 140559
Co-fractionation Mus musculus
4 Slc44a1 100434
Co-fractionation Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
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