Gene description for Parp1
Gene name poly (ADP-ribose) polymerase family, member 1
Gene symbol Parp1
Other names/aliases 5830444G22Rik
AI893648
ARTD1
Adprp
Adprt1
C80510
PARP
PPOL
parp-1
sPARP-1
Species Mus musculus
 Database cross references - Parp1
ExoCarta ExoCarta_11545
Vesiclepedia VP_11545
Entrez Gene 11545
 Parp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Parp1
Molecular Function
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IDA
    chromatin binding GO:0003682 ISO
    damaged DNA binding GO:0003684 ISO
    damaged DNA binding GO:0003684 ISS
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IBA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IDA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IMP
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 ISO
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    nucleotidyltransferase activity GO:0016779 IEA
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    nuclear estrogen receptor binding GO:0030331 ISO
    nucleosome binding GO:0031491 ISO
    nucleosome binding GO:0031491 ISS
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    histone deacetylase binding GO:0042826 ISO
    NAD binding GO:0051287 IEA
    NAD binding GO:0051287 ISO
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    R-SMAD binding GO:0070412 ISO
    NAD DNA ADP-ribosyltransferase activity GO:0140294 ISO
    transcription regulator activator activity GO:0140537 ISO
    NAD+-protein-serine ADP-ribosyltransferase activity GO:0140805 ISO
    NAD+-protein-serine ADP-ribosyltransferase activity GO:0140805 ISS
    NAD+-protein-aspartate ADP-ribosyltransferase activity GO:0140806 IDA
    NAD+-protein-aspartate ADP-ribosyltransferase activity GO:0140806 ISO
    NAD+-protein-glutamate ADP-ribosyltransferase activity GO:0140807 IDA
    NAD+-protein-glutamate ADP-ribosyltransferase activity GO:0140807 IMP
    NAD+-protein-glutamate ADP-ribosyltransferase activity GO:0140807 ISO
    NAD+-protein-tyrosine ADP-ribosyltransferase activity GO:0140808 ISO
    NAD+-protein-tyrosine ADP-ribosyltransferase activity GO:0140808 ISS
    NAD+-protein-histidine ADP-ribosyltransferase activity GO:0140815 ISO
    NAD+-protein-histidine ADP-ribosyltransferase activity GO:0140815 ISS
    NAD+-histone H2BS6 serine ADP-ribosyltransferase activity GO:0140816 IEA
    NAD+-histone H2BS6 serine ADP-ribosyltransferase activity GO:0140816 ISO
    NAD+-histone H3S10 serine ADP-ribosyltransferase activity GO:0140817 IEA
    NAD+-histone H3S10 serine ADP-ribosyltransferase activity GO:0140817 ISO
    NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity GO:0140822 IDA
    NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity GO:0140844 IEA
    NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity GO:0140867 IEA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IBA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IDA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    telomere maintenance GO:0000723 IMP
    DNA metabolic process GO:0006259 IMP
    DNA repair GO:0006281 IDA
    DNA repair GO:0006281 ISO
    DNA repair GO:0006281 TAS
    base-excision repair GO:0006284 IMP
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 IGI
    double-strand break repair GO:0006302 ISO
    double-strand break repair GO:0006302 ISS
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISS
    mitochondrion organization GO:0007005 IMP
    mitochondrion organization GO:0007005 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISO
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISS
    carbohydrate biosynthetic process GO:0016051 ISO
    protein autoprocessing GO:0016540 ISO
    signal transduction involved in regulation of gene expression GO:0023019 ISO
    DNA ADP-ribosylation GO:0030592 ISO
    DNA ADP-ribosylation GO:0030592 ISS
    mitochondrial DNA metabolic process GO:0032042 ISO
    negative regulation of interleukin-17 production GO:0032700 IMP
    positive regulation of DNA-templated transcription, elongation GO:0032786 ISO
    cellular response to insulin stimulus GO:0032869 ISO
    regulation of protein localization GO:0032880 ISO
    positive regulation of intracellular estrogen receptor signaling pathway GO:0033148 ISO
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 ISO
    cellular response to oxidative stress GO:0034599 ISO
    cellular response to UV GO:0034644 ISO
    protein modification process GO:0036211 ISO
    regulation of growth rate GO:0040009 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    mitochondrial DNA repair GO:0043504 ISO
    innate immune response GO:0045087 IEA
    regulation of circadian sleep/wake cycle, non-REM sleep GO:0045188 IMP
    negative regulation of innate immune response GO:0045824 IMP
    negative regulation of innate immune response GO:0045824 ISO
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    decidualization GO:0046697 IMP
    behavioral response to cocaine GO:0048148 IMP
    regulation of catalytic activity GO:0050790 ISO
    voluntary musculoskeletal movement GO:0050882 IGI
    positive regulation of mitochondrial depolarization GO:0051901 ISO
    positive regulation of SMAD protein signal transduction GO:0060391 ISO
    positive regulation of necroptotic process GO:0060545 IDA
    protein poly-ADP-ribosylation GO:0070212 IDA
    protein poly-ADP-ribosylation GO:0070212 IDA
    protein poly-ADP-ribosylation GO:0070212 ISO
    protein auto-ADP-ribosylation GO:0070213 ISO
    protein auto-ADP-ribosylation GO:0070213 ISS
    protein localization to chromatin GO:0071168 ISO
    cellular response to superoxide GO:0071451 IDA
    replication fork reversal GO:0071932 ISO
    replication fork reversal GO:0071932 ISS
    negative regulation of cGAS/STING signaling pathway GO:0160049 ISO
    positive regulation of protein localization to nucleus GO:1900182 ISO
    regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903376 ISO
    regulation of single strand break repair GO:1903516 IMP
    positive regulation of single strand break repair GO:1903518 IBA
    positive regulation of single strand break repair GO:1903518 IGI
    positive regulation of single strand break repair GO:1903518 ISO
    negative regulation of adipose tissue development GO:1904178 IDA
    negative regulation of telomere maintenance via telomere lengthening GO:1904357 IMP
    cellular response to amyloid-beta GO:1904646 ISO
    positive regulation of myofibroblast differentiation GO:1904762 ISO
    regulation of base-excision repair GO:1905051 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISS
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    ATP generation from poly-ADP-D-ribose GO:1990966 ISO
    ATP generation from poly-ADP-D-ribose GO:1990966 ISS
    negative regulation of ATP biosynthetic process GO:2001170 ISO
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 TAS
    nuclear envelope GO:0005635 ISO
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    transcription regulator complex GO:0005667 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    nuclear body GO:0016604 ISO
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    protein-DNA complex GO:0032993 ISO
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    nuclear replication fork GO:0043596 ISO
    nuclear replication fork GO:0043596 ISS
    site of DNA damage GO:0090734 IDA
    site of DNA damage GO:0090734 ISO
    site of DNA damage GO:0090734 ISO
    site of DNA damage GO:0090734 ISS
 Experiment description of studies that identified Parp1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Parp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Affinity Capture-Western Mus musculus
2 Mycbp2 105689
Affinity Capture-MS Mus musculus
3 St18  
Co-fractionation Mus musculus
4 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
5 Nfyb  
Co-fractionation Mus musculus
6 Xrcc5  
Co-fractionation Mus musculus
7 Faf1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
8 Kcna3  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Tfe3  
Affinity Capture-MS Mus musculus
11 Dnmt1 13433
Affinity Capture-Western Mus musculus
12 Cenpb  
Two-hybrid Mus musculus
13 Sox2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
14 Ogg1  
Reconstituted Complex Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
15 Aurkb  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Far Western Mus musculus
Biochemical Activity Mus musculus
16 Otud5  
Affinity Capture-MS Mus musculus
17 Nanog  
Affinity Capture-MS Mus musculus
18 Casp3 12367
Biochemical Activity Mus musculus
19 Top2a 21973
Co-fractionation Mus musculus
20 Brca1  
Synthetic Lethality Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Top1 21969
Co-fractionation Mus musculus
23 Uhrf1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
24 Trim33  
Co-localization Mus musculus
25 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Parp1 is involved
No pathways found





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