Gene description for Ap1b1
Gene name adaptor protein complex AP-1, beta 1 subunit
Gene symbol Ap1b1
Other names/aliases Adtb1
b2b1660Clo
Species Mus musculus
 Database cross references - Ap1b1
ExoCarta ExoCarta_11764
Vesiclepedia VP_11764
Entrez Gene 11764
UniProt O35643  
 Ap1b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap1b1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    clathrin binding GO:0030276 IEA
    protein serine/threonine kinase binding GO:0120283 ISO
Biological Process
    kidney development GO:0001822 IMP
    intracellular protein transport GO:0006886 TAS
    determination of left/right symmetry GO:0007368 IMP
    heart development GO:0007507 IMP
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    vesicle-mediated transport GO:0016192 TAS
    clathrin coat assembly GO:0048268 ISO
    platelet dense granule organization GO:0060155 NAS
    basolateral protein secretion GO:0110010 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    lysosomal membrane GO:0005765 NAS
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 TAS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    synaptic vesicle GO:0008021 EXP
    synaptic vesicle GO:0008021 IDA
    AP-1 adaptor complex GO:0030121 NAS
    trans-Golgi network membrane GO:0032588 NAS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Ap1b1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap1b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lnx1  
Affinity Capture-MS Mus musculus
2 HTT 3064
Affinity Capture-MS Homo sapiens
3 SNCA 6622
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Cltc 67300
Co-fractionation Mus musculus
6 Foxp3  
Affinity Capture-MS Mus musculus
7 Crebbp  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
8 Atg5  
Affinity Capture-MS Mus musculus
9 Snap91  
Co-fractionation Mus musculus
10 Agap2  
Affinity Capture-MS Mus musculus
11 ATM 472
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 Trmt61a  
Co-fractionation Mus musculus
13 Reps1  
Co-fractionation Mus musculus
14 Eps15 13858
Co-fractionation Mus musculus
15 Cyfip1 20430
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
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