Gene description for CLCN4
Gene name chloride channel, voltage-sensitive 4
Gene symbol CLCN4
Other names/aliases CLC4
ClC-4
ClC-4A
Species Homo sapiens
 Database cross references - CLCN4
ExoCarta ExoCarta_1183
Vesiclepedia VP_1183
Entrez Gene 1183
HGNC 2022
MIM 302910
UniProt P51793  
 CLCN4 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CLCN4
Molecular Function
    voltage-gated chloride channel activity GO:0005247 IBA
    chloride channel activity GO:0005254 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    antiporter activity GO:0015297 IDA
    antiporter activity GO:0015297 IMP
    antiporter activity GO:0015297 TAS
Biological Process
    chloride transport GO:0006821 IDA
    chloride transport GO:0006821 IMP
    monoatomic ion transmembrane transport GO:0034220 TAS
    chloride transmembrane transport GO:1902476 IEA
    non-motile cilium assembly GO:1905515 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    early endosome GO:0005769 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IBA
    plasma membrane GO:0005886 IBA
    synaptic vesicle GO:0008021 IBA
    endosome membrane GO:0010008 IDA
    endosome membrane GO:0010008 TAS
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IEA
    ciliary base GO:0097546 IEA
 Experiment description of studies that identified CLCN4 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CLCN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM9 252839
Affinity Capture-MS Homo sapiens
2 TMEM9B 56674
Affinity Capture-MS Homo sapiens
3 MTAP 4507
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which CLCN4 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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