Gene description for SLC15A4
Gene name solute carrier family 15 (oligopeptide transporter), member 4
Gene symbol SLC15A4
Other names/aliases FP12591
PHT1
PTR4
Species Homo sapiens
 Database cross references - SLC15A4
ExoCarta ExoCarta_121260
Vesiclepedia VP_121260
Entrez Gene 121260
HGNC 23090
MIM 615806
UniProt Q8N697  
 SLC15A4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC15A4
Molecular Function
    L-histidine transmembrane transporter activity GO:0005290 IBA
    L-histidine transmembrane transporter activity GO:0005290 IDA
    protein binding GO:0005515 IPI
    peptide:proton symporter activity GO:0015333 IDA
    peptide:proton symporter activity GO:0015333 TAS
    peptidoglycan transmembrane transporter activity GO:0015647 IDA
    dipeptide transmembrane transporter activity GO:0071916 IBA
    dipeptide transmembrane transporter activity GO:0071916 IDA
Biological Process
    monoatomic ion transport GO:0006811 TAS
    protein transport GO:0015031 IEA
    histidine transport GO:0015817 IBA
    peptidoglycan transport GO:0015835 IDA
    mast cell homeostasis GO:0033023 ISS
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 IMP
    positive regulation of toll-like receptor 8 signaling pathway GO:0034161 IMP
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 IMP
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IDA
    regulation of isotype switching to IgG isotypes GO:0048302 ISS
    regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0070424 ISS
    positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway GO:0070430 ISS
    positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070434 ISS
    L-histidine transmembrane export from vacuole GO:0089708 IEA
    dipeptide import across plasma membrane GO:0140206 IBA
    dipeptide import across plasma membrane GO:0140206 IDA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IBA
    early endosome membrane GO:0031901 IEA
    specific granule membrane GO:0035579 TAS
    endolysosome membrane GO:0036020 IDA
    endolysosome membrane GO:0036020 TAS
 Experiment description of studies that identified SLC15A4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC15A4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HTR3B  
Affinity Capture-MS Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LGALS9 3965
Affinity Capture-MS Homo sapiens
5 CLCN7 1186
Affinity Capture-MS Homo sapiens
6 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SLC30A2  
Affinity Capture-MS Homo sapiens
8 CHRNA4  
Affinity Capture-MS Homo sapiens
9 SLC22A2 6582
Affinity Capture-MS Homo sapiens
10 SIDT2 51092
Affinity Capture-MS Homo sapiens
11 LRRC55  
Affinity Capture-MS Homo sapiens
12 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
14 TSPAN15 23555
Affinity Capture-MS Homo sapiens
15 CXorf21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GPR35  
Two-hybrid Homo sapiens
18 SCN4A  
Affinity Capture-MS Homo sapiens
19 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SLC5A4  
Affinity Capture-MS Homo sapiens
21 KIF23 9493
Affinity Capture-MS Homo sapiens
22 CMTM5  
Affinity Capture-MS Homo sapiens
23 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TNFRSF10C  
Affinity Capture-MS Homo sapiens
26 UPK1A 11045
Affinity Capture-MS Homo sapiens
27 DRD2  
Two-hybrid Homo sapiens
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