Gene description for ADH1A
Gene name alcohol dehydrogenase 1A (class I), alpha polypeptide
Gene symbol ADH1A
Other names/aliases ADH1
Species Homo sapiens
 Database cross references - ADH1A
ExoCarta ExoCarta_124
Vesiclepedia VP_124
Entrez Gene 124
HGNC 249
MIM 103700
UniProt P07327  
 ADH1A identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADH1A
Molecular Function
    alcohol dehydrogenase (NAD+) activity GO:0004022 IBA
    alcohol dehydrogenase (NAD+) activity GO:0004022 IDA
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    butanol dehydrogenase (NAD+) activity GO:1990362 IEA
Biological Process
    alcohol metabolic process GO:0006066 IDA
    alcohol metabolic process GO:0006066 NAS
    retinol metabolic process GO:0042572 IBA
    retinoic acid metabolic process GO:0042573 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ADH1A in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADH1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CSNK2A2 1459
Two-hybrid Homo sapiens
2 HPN  
Affinity Capture-MS Homo sapiens
3 ADH1B 125
Affinity Capture-MS Homo sapiens
4 HADH 3033
Two-hybrid Homo sapiens
View the network image/svg+xml



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