Experiment description of studies that identified FAM168B in sEVs

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for FAM168B

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 TIAL1 7073 Two-hybrid Homo sapiens 3 ARID5A   Two-hybrid Homo sapiens 4 FOXH1   Two-hybrid Homo sapiens 5 SPAG8   Two-hybrid Homo sapiens 6 MYCN   Affinity Capture-MS Homo sapiens 7 PRR35   Two-hybrid Homo sapiens 8 BAG3 9531 Two-hybrid Homo sapiens 9 AGXT   Two-hybrid Homo sapiens 10 HSF4   Two-hybrid Homo sapiens 11 ROR2 4920 Two-hybrid Homo sapiens 12 WDR25   Two-hybrid Homo sapiens 13 CSTF2T   Two-hybrid Homo sapiens 14 VPS37C 55048 Two-hybrid Homo sapiens 15 EGLN3   Two-hybrid Homo sapiens 16 UBAP2 55833 Two-hybrid Homo sapiens 17 AES 166 Two-hybrid Homo sapiens 18 SNRPB 6628 Two-hybrid Homo sapiens 19 POGZ 23126 Two-hybrid Homo sapiens 20 NEDD9 4739 Two-hybrid Homo sapiens 21 MEX3A   Affinity Capture-RNA Homo sapiens 22 VEZF1   Two-hybrid Homo sapiens 23 FOXI1   Two-hybrid Homo sapiens 24 TCF7L2   Two-hybrid Homo sapiens 25 FAM46B   Two-hybrid Homo sapiens 26 TLX3   Two-hybrid Homo sapiens 27 POM121   Two-hybrid Homo sapiens 28 ZIC1   Two-hybrid Homo sapiens 29 MYOZ3   Two-hybrid Homo sapiens 30 RHOXF2   Two-hybrid Homo sapiens 31 CSTF2 1478 Two-hybrid Homo sapiens 32 OTX1   Two-hybrid Homo sapiens 33 ZC3H10   Two-hybrid Homo sapiens 34 HOXB9   Two-hybrid Homo sapiens 35 VGLL3   Two-hybrid Homo sapiens 36 KRTAP26-1   Two-hybrid Homo sapiens 37 MSRB3 253827 Two-hybrid Homo sapiens 38 GLIS2   Two-hybrid Homo sapiens 39 SAMD7   Two-hybrid Homo sapiens 40 PPP1R32   Two-hybrid Homo sapiens 41 SNRPC 6631 Two-hybrid Homo sapiens 42 DDX58 23586 Affinity Capture-RNA Homo sapiens 43 VENTX   Two-hybrid Homo sapiens 44 FAM222B   Two-hybrid Homo sapiens 45 MED25   Two-hybrid Homo sapiens 46 FAM103A1   Two-hybrid Homo sapiens 47 GPS2   Two-hybrid Homo sapiens View the network

Pathways in which FAM168B is involved

No pathways found