Gene description for Dag1
Gene name dystroglycan 1
Gene symbol Dag1
Other names/aliases D9Wsu13e
DG
Dp427
Dp71
Species Mus musculus
 Database cross references - Dag1
ExoCarta ExoCarta_13138
Vesiclepedia VP_13138
Entrez Gene 13138
UniProt Q62165  
 Dag1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dag1
Molecular Function
    virus receptor activity GO:0001618 IEA
    virus receptor activity GO:0001618 ISO
    dystroglycan binding GO:0002162 IPI
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 IEA
    structural constituent of muscle GO:0008307 ISO
    tubulin binding GO:0015631 IEA
    tubulin binding GO:0015631 ISO
    vinculin binding GO:0017166 IEA
    vinculin binding GO:0017166 ISO
    SH2 domain binding GO:0042169 IEA
    SH2 domain binding GO:0042169 ISO
    laminin binding GO:0043236 IBA
    laminin binding GO:0043236 IDA
    laminin binding GO:0043236 ISO
    laminin-1 binding GO:0043237 IEA
    laminin-1 binding GO:0043237 ISO
    protein-containing complex binding GO:0044877 IDA
    alpha-actinin binding GO:0051393 IEA
    alpha-actinin binding GO:0051393 ISO
Biological Process
    positive regulation of cell-matrix adhesion GO:0001954 IEA
    positive regulation of cell-matrix adhesion GO:0001954 ISO
    morphogenesis of an epithelium GO:0002009 IBA
    morphogenesis of an epithelial sheet GO:0002011 IMP
    heart morphogenesis GO:0003007 IMP
    membrane protein ectodomain proteolysis GO:0006509 IEA
    membrane protein ectodomain proteolysis GO:0006509 ISO
    axon guidance GO:0007411 IBA
    heart development GO:0007507 IMP
    regulation of gastrulation GO:0010470 IEA
    regulation of gastrulation GO:0010470 ISO
    regulation of epithelial to mesenchymal transition GO:0010717 IEA
    regulation of epithelial to mesenchymal transition GO:0010717 ISO
    Schwann cell development GO:0014044 IMP
    response to muscle activity GO:0014850 IDA
    response to denervation involved in regulation of muscle adaptation GO:0014894 IEA
    protein transport GO:0015031 IDA
    muscle attachment GO:0016203 IBA
    calcium-dependent cell-matrix adhesion GO:0016340 IEA
    calcium-dependent cell-matrix adhesion GO:0016340 ISO
    nerve development GO:0021675 IBA
    nerve maturation GO:0021682 IMP
    myelination in peripheral nervous system GO:0022011 IMP
    negative regulation of cell migration GO:0030336 IEA
    negative regulation of cell migration GO:0030336 ISO
    axon regeneration GO:0031103 IEA
    positive regulation of myelination GO:0031643 IEA
    positive regulation of myelination GO:0031643 ISO
    microtubule anchoring GO:0034453 IEA
    microtubule anchoring GO:0034453 ISO
    skeletal muscle tissue regeneration GO:0043403 IEA
    negative regulation of MAPK cascade GO:0043409 IEA
    negative regulation of MAPK cascade GO:0043409 ISO
    response to peptide hormone GO:0043434 IEA
    symbiont entry into host cell GO:0046718 IEA
    regulation of synaptic plasticity GO:0048167 IMP
    positive regulation of oligodendrocyte differentiation GO:0048714 IEA
    positive regulation of oligodendrocyte differentiation GO:0048714 ISO
    regulation of synapse organization GO:0050807 IDA
    regulation of synapse organization GO:0050807 IMP
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    angiogenesis involved in wound healing GO:0060055 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IMP
    branching involved in salivary gland morphogenesis GO:0060445 IMP
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to mechanical stimulus GO:0071260 ISO
    cellular response to cholesterol GO:0071397 IEA
    cellular response to organic cyclic compound GO:0071407 IDA
    commissural neuron axon guidance GO:0071679 IMP
    basement membrane organization GO:0071711 IMP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IDA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IMP
    retrograde trans-synaptic signaling by trans-synaptic protein complex GO:0098942 IDA
    retrograde trans-synaptic signaling by trans-synaptic protein complex GO:0098942 IMP
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IEA
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 ISO
Subcellular Localization
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IBA
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IEA
    adherens junction GO:0005912 ISO
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    focal adhesion GO:0005925 ISO
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    dystrophin-associated glycoprotein complex GO:0016010 ISO
    dystroglycan complex GO:0016011 IBA
    dystroglycan complex GO:0016011 IDA
    dystroglycan complex GO:0016011 IMP
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    filopodium GO:0030175 IEA
    filopodium GO:0030175 ISO
    node of Ranvier GO:0033268 IMP
    nuclear periphery GO:0034399 IEA
    nuclear periphery GO:0034399 ISO
    sarcolemma GO:0042383 IBA
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    costamere GO:0043034 IEA
    costamere GO:0043034 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    plasma membrane raft GO:0044853 IEA
    plasma membrane raft GO:0044853 ISO
    membrane raft GO:0045121 IDA
    postsynaptic membrane GO:0045211 IEA
    contractile ring GO:0070938 IEA
    contractile ring GO:0070938 ISO
    photoreceptor ribbon synapse GO:0098684 IDA
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
    postsynaptic cytosol GO:0099524 IEA
    postsynaptic cytosol GO:0099524 ISO
 Experiment description of studies that identified Dag1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dag1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sgcd  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
2 Sgcb  
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Utrn  
Affinity Capture-Western Mus musculus
5 Drp2  
Co-fractionation Mus musculus
6 Sgca  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Co-fractionation Mus musculus
7 Cav3  
Co-fractionation Mus musculus
8 Snta1  
Co-fractionation Mus musculus
9 Vcl 22330
Co-fractionation Mus musculus
10 Rapsn  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
11 Egflam  
Co-fractionation Mus musculus
12 Dmd  
Affinity Capture-MS Mus musculus
Protein-peptide Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
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