Experiment description of studies that identified PACRG in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for PACRG

Protein Interactor ExoCarta ID Identification method PubMed Species 1 STUB1 10273 Affinity Capture-Western Homo sapiens 2 CCT7 10574 Affinity Capture-MS Homo sapiens 3 CCT6A 908 Affinity Capture-MS Homo sapiens 4 CCT5 22948 Affinity Capture-MS Homo sapiens 5 MEIG1   Two-hybrid Homo sapiens 6 TUBA1A 7846 Affinity Capture-MS Homo sapiens 7 KLHL36   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 TCP1 6950 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 9 GPR37   Affinity Capture-Western Homo sapiens 10 DNAJA1 3301 Affinity Capture-Western Homo sapiens 11 CCDC40   Affinity Capture-MS Homo sapiens 12 HSPA4 3308 Affinity Capture-Western Homo sapiens 13 CCT3 7203 Affinity Capture-MS Homo sapiens 14 CCT4 10575 Affinity Capture-MS Homo sapiens 15 TUBB 203068 Affinity Capture-MS Homo sapiens 16 PARK2   Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 17 TP53 7157 Synthetic Growth Defect Homo sapiens 18 HSP90AA1 3320 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 19 APP 351 Reconstituted Complex Homo sapiens 20 CCT2 10576 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 21 VCP 7415 Affinity Capture-Western Homo sapiens View the network

Pathways in which PACRG is involved

No pathways found