Gene description for Glul
Gene name glutamate-ammonia ligase (glutamine synthetase)
Gene symbol Glul
Other names/aliases GS
Glns
Species Mus musculus
 Database cross references - Glul
ExoCarta ExoCarta_14645
Vesiclepedia VP_14645
Entrez Gene 14645
UniProt P15105  
 Glul identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Glul
Molecular Function
    magnesium ion binding GO:0000287 ISO
    glutamine synthetase activity GO:0004356 IBA
    glutamine synthetase activity GO:0004356 ISO
    glutamine synthetase activity GO:0004356 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    nickel cation binding GO:0016151 ISO
    glutamate binding GO:0016595 ISO
    protein-cysteine S-palmitoyltransferase activity GO:0019706 ISO
    protein-cysteine S-palmitoyltransferase activity GO:0019706 ISS
    manganese ion binding GO:0030145 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    dynein light chain binding GO:0045503 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    glutamate metabolic process GO:0006536 ISO
    glutamine biosynthetic process GO:0006542 IBA
    glutamine biosynthetic process GO:0006542 IEA
    glutamine biosynthetic process GO:0006542 ISO
    cell population proliferation GO:0008283 IEA
    cell population proliferation GO:0008283 ISO
    cellular response to starvation GO:0009267 IEP
    response to glucose GO:0009749 IMP
    regulation of endothelial cell migration GO:0010594 IMP
    regulation of endothelial cell migration GO:0010594 ISO
    protein palmitoylation GO:0018345 ISO
    protein palmitoylation GO:0018345 ISS
    ammonia assimilation cycle GO:0019676 ISO
    positive regulation of insulin secretion GO:0032024 ISO
    ribosome biogenesis GO:0042254 IEA
    ribosome biogenesis GO:0042254 ISO
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    positive regulation of synaptic transmission, glutamatergic GO:0051968 ISO
    regulation of sprouting angiogenesis GO:1903670 IMP
    regulation of sprouting angiogenesis GO:1903670 ISO
    regulation of protein localization to nucleolus GO:1904749 IEA
    regulation of protein localization to nucleolus GO:1904749 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    protein-containing complex GO:0032991 ISO
    cell projection GO:0042995 ISO
    perikaryon GO:0043204 ISO
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 ISO
    axon terminus GO:0043679 ISO
    cell body GO:0044297 IDA
    glial cell projection GO:0097386 IDA
 Experiment description of studies that identified Glul in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Glul
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
2 SNCA 6622
Affinity Capture-MS Homo sapiens
3 Cnksr2  
Affinity Capture-MS Mus musculus
4 Cyfip1 20430
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Agap2  
Affinity Capture-MS Mus musculus
7 MAPT  
Reconstituted Complex Homo sapiens
8 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Tnik  
Affinity Capture-MS Mus musculus
10 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
11 HSD17B10 3028
Affinity Capture-MS Homo sapiens
12 Huwe1  
Affinity Capture-MS Mus musculus
13 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here