Gene description for Gna13
Gene name guanine nucleotide binding protein, alpha 13
Gene symbol Gna13
Other names/aliases AU024132
AU043124
Galpha13
Species Mus musculus
 Database cross references - Gna13
ExoCarta ExoCarta_14674
Vesiclepedia VP_14674
Entrez Gene 14674
UniProt P27601  
 Gna13 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gna13
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISA
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    D5 dopamine receptor binding GO:0031752 IBA
    D5 dopamine receptor binding GO:0031752 IEA
    D5 dopamine receptor binding GO:0031752 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 IMP
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    in utero embryonic development GO:0001701 IGI
    G protein-coupled receptor signaling pathway GO:0007186 ISA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IGI
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IDA
    regulation of blood pressure GO:0008217 IGI
    regulation of cell shape GO:0008360 IDA
    regulation of fibroblast migration GO:0010762 IMP
    cell differentiation GO:0030154 IDA
    platelet activation GO:0030168 IMP
    intracellular signal transduction GO:0035556 IDA
    regulation of postsynapse assembly GO:0150052 EXP
    regulation of postsynapse assembly GO:0150052 IDA
    regulation of postsynapse assembly GO:0150052 IEP
    regulation of postsynapse assembly GO:0150052 IMP
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 ISO
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 ISA
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 TAS
    brush border membrane GO:0031526 IBA
    brush border membrane GO:0031526 IEA
    brush border membrane GO:0031526 ISO
    melanosome GO:0042470 IEA
    postsynapse GO:0098794 EXP
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IEP
    postsynapse GO:0098794 IMP
 Experiment description of studies that identified Gna13 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gna13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
2 Atxn1  
Proximity Label-MS Mus musculus
3 Agap2  
Affinity Capture-MS Mus musculus
4 Ric8  
Co-fractionation Mus musculus
5 Cdh1 12550
Affinity Capture-Western Mus musculus
6 Ptk2b  
Co-fractionation Mus musculus
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