Gene description for Dnaja1
Gene name DnaJ (Hsp40) homolog, subfamily A, member 1
Gene symbol Dnaja1
Other names/aliases Hsj2
Nedd7
Species Mus musculus
 Database cross references - Dnaja1
ExoCarta ExoCarta_15502
Vesiclepedia VP_15502
Entrez Gene 15502
UniProt P63037  
 Dnaja1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnaja1
Molecular Function
    G protein-coupled receptor binding GO:0001664 IEA
    G protein-coupled receptor binding GO:0001664 ISO
    ATPase activator activity GO:0001671 ISO
    ATPase activator activity GO:0001671 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    Hsp70 protein binding GO:0030544 ISO
    Hsp70 protein binding GO:0030544 ISS
    Tat protein binding GO:0030957 IEA
    Tat protein binding GO:0030957 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    metal ion binding GO:0046872 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    unfolded protein binding GO:0051082 IEA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
    protein-folding chaperone binding GO:0051087 ISS
    C3HC4-type RING finger domain binding GO:0055131 IEA
    C3HC4-type RING finger domain binding GO:0055131 ISO
Biological Process
    protein folding GO:0006457 IEA
    spermatogenesis GO:0007283 IMP
    response to heat GO:0009408 IEA
    flagellated sperm motility GO:0030317 IMP
    androgen receptor signaling pathway GO:0030521 IMP
    negative regulation of protein ubiquitination GO:0031397 IEA
    negative regulation of protein ubiquitination GO:0031397 ISO
    protein refolding GO:0042026 IBA
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of apoptotic process GO:0043065 ISS
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of JUN kinase activity GO:0043508 ISO
    negative regulation of JUN kinase activity GO:0043508 ISS
    regulation of protein transport GO:0051223 ISO
    regulation of protein transport GO:0051223 ISS
    protein localization to mitochondrion GO:0070585 ISO
    protein localization to mitochondrion GO:0070585 ISS
    negative regulation of establishment of protein localization to mitochondrion GO:1903748 IEA
    negative regulation of establishment of protein localization to mitochondrion GO:1903748 ISO
    negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway GO:1905259 IEA
    negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway GO:1905259 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IEA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Dnaja1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnaja1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Atxn3  
Affinity Capture-Western Mus musculus
3 Gpr37  
Co-fractionation Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 GLIS3  
Two-hybrid Homo sapiens
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Srrm2  
Co-fractionation Mus musculus
9 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
10 SOD1 6647
Affinity Capture-MS Homo sapiens
11 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
12 Iqcb1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
13 Nanog  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Lrrk2  
Co-fractionation Mus musculus
16 Tsc1  
Affinity Capture-MS Mus musculus
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