Experiment description of studies that identified Il17ra in sEVs |
| 1 |
| Experiment ID |
520 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Cd9
|
Enriched markers |
|
✔
Golga2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34112803
|
| Organism |
Mus musculus |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
EO771 |
| Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting Mass spectometry |
|
|
| 2 |
| Experiment ID |
521 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Cd9
|
Enriched markers |
|
✔
Golga2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34112803
|
| Organism |
Mus musculus |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
PyMT |
| Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting Mass spectometry |
|
|