Gene description for OTUD7A
Gene name OTU deubiquitinase 7A
Gene symbol OTUD7A
Other names/aliases C15orf16
C16ORF15
CEZANNE2
OTUD7
Species Homo sapiens
 Database cross references - OTUD7A
ExoCarta ExoCarta_161725
Vesiclepedia VP_161725
Entrez Gene 161725
HGNC 20718
MIM 612024
UniProt Q8TE49  
 OTUD7A identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for OTUD7A
Molecular Function
    DNA binding GO:0003677 IEA
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    zinc ion binding GO:0008270 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IBA
Biological Process
    protein deubiquitination GO:0016579 TAS
    protein K11-linked deubiquitination GO:0035871 IDA
    protein deubiquitination involved in ubiquitin-dependent protein catabolic process GO:0071947 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified OTUD7A in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for OTUD7A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 AURKA 6790
Affinity Capture-Western Homo sapiens
3 OTUD5 55593
Affinity Capture-MS Homo sapiens
4 TRAF6 7189
Affinity Capture-Western Homo sapiens
5 UBC 7316
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
6 OTUD7B 56957
Affinity Capture-MS Homo sapiens
7 TRIM21 6737
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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