Gene description for Kpna2
Gene name karyopherin (importin) alpha 2
Gene symbol Kpna2
Other names/aliases 2410044B12Rik
IPOA1
PTAC58
Rch1
Species Mus musculus
 Database cross references - Kpna2
ExoCarta ExoCarta_16647
Vesiclepedia VP_16647
Entrez Gene 16647
UniProt P52293  
 Kpna2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kpna2
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    nuclear localization sequence binding GO:0008139 ISO
    histone deacetylase binding GO:0042826 ISO
    nuclear import signal receptor activity GO:0061608 IBA
    nuclear import signal receptor activity GO:0061608 IDA
    nuclear import signal receptor activity GO:0061608 ISO
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    protein import into nucleus GO:0006606 IDA
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISS
    NLS-bearing protein import into nucleus GO:0006607 IBA
    NLS-bearing protein import into nucleus GO:0006607 ISO
    positive regulation of type I interferon production GO:0032481 ISO
    entry of viral genome into host nucleus through nuclear pore complex via importin GO:0075506 IMP
    entry of viral genome into host nucleus through nuclear pore complex via importin GO:0075506 ISO
    postsynapse to nucleus signaling pathway GO:0099527 IDA
    postsynapse to nucleus signaling pathway GO:0099527 IMP
    positive regulation of viral life cycle GO:1903902 IMP
    positive regulation of viral life cycle GO:1903902 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 NAS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IDA
    postsynaptic density GO:0014069 IDA
    postsynaptic density GO:0014069 IEP
    nuclear membrane GO:0031965 ISO
    NLS-dependent protein nuclear import complex GO:0042564 ISO
    host cell GO:0043657 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Kpna2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kpna2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elf5  
Affinity Capture-MS Mus musculus
2 Rab11fip3  
Co-fractionation Mus musculus
3 CBX1 10951
Reconstituted Complex Homo sapiens
4 Pou5f1  
Affinity Capture-MS Mus musculus
5 Cry2  
Affinity Capture-Western Mus musculus
6 TP53BP1 7158
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
7 Chd8  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
8 Cdkn1b  
Co-fractionation Mus musculus
9 Kpnb1 16211
Co-fractionation Mus musculus
10 Mroh2a  
Co-fractionation Mus musculus
11 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
12 Acly 104112
Co-fractionation Mus musculus
13 Ngly1  
Two-hybrid Mus musculus
14 Fzr1  
Affinity Capture-MS Mus musculus
15 Pcyt1a  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
FRET Mus musculus
16 TRIM28 10155
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
17 Nanog  
Affinity Capture-MS Mus musculus
18 Kpna6  
Co-fractionation Mus musculus
19 SART3 9733
Co-crystal Structure Homo sapiens
20 Kpna4  
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 LEF1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 Fancd2  
Affinity Capture-MS Mus musculus
24 Rag1  
Two-hybrid Mus musculus
Co-fractionation Mus musculus
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