Gene description for PAPD4
Gene name PAP associated domain containing 4
Gene symbol PAPD4
Other names/aliases GLD2
TUT2
Species Homo sapiens
 Database cross references - PAPD4
ExoCarta ExoCarta_167153
Vesiclepedia VP_167153
Entrez Gene 167153
HGNC 26776
MIM 614121
UniProt Q6PIY7  
 PAPD4 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PAPD4
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    poly(A) RNA polymerase activity GO:1990817 IBA
    poly(A) RNA polymerase activity GO:1990817 IDA
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    RNA 3'-end processing GO:0031123 IBA
    mRNA 3'-end processing GO:0031124 IDA
    histone mRNA catabolic process GO:0071044 IMP
    target-directed miRNA degradation GO:0140958 IEA
    negative regulation of miRNA catabolic process GO:2000626 IDA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nuclear RNA-directed RNA polymerase complex GO:0031380 IC
 Experiment description of studies that identified PAPD4 in sEVs
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PAPD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CADPS  
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
3 KRTAP6-3  
Two-hybrid Homo sapiens
4 CALCOCO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 CUL3 8452
Affinity Capture-MS Homo sapiens
6 NANOG  
Affinity Capture-MS Homo sapiens
7 RBPMS 11030
Two-hybrid Homo sapiens
8 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
9 OGT 8473
Reconstituted Complex Homo sapiens
10 ILF3 3609
Affinity Capture-MS Homo sapiens
11 TAF1  
Affinity Capture-MS Homo sapiens
12 RBM4B  
Affinity Capture-MS Homo sapiens
13 BCCIP 56647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PAPD4 is involved
No pathways found





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