Gene description for Lmnb1
Gene name lamin B1
Gene symbol Lmnb1
Other names/aliases -
Species Mus musculus
 Database cross references - Lmnb1
ExoCarta ExoCarta_16906
Vesiclepedia VP_16906
Entrez Gene 16906
UniProt P14733  
 Lmnb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lmnb1
Molecular Function
    double-stranded DNA binding GO:0003690 IDA
    structural constituent of cytoskeleton GO:0005200 IBA
    JUN kinase binding GO:0008432 NAS
    phospholipase binding GO:0043274 IPI
    structural constituent of nuclear lamina GO:0160123 IDA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    nuclear envelope organization GO:0006998 IBA
    nuclear envelope organization GO:0006998 ISO
    nuclear envelope organization GO:0006998 ISS
    nuclear migration GO:0007097 IBA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 NAS
    heterochromatin formation GO:0031507 IBA
    positive regulation of JNK cascade GO:0046330 NAS
    nuclear pore localization GO:0051664 IBA
    protein localization to nuclear envelope GO:0090435 IBA
    cellular response to L-glutamate GO:1905232 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 TAS
    nuclear inner membrane GO:0005637 IDA
    lamin filament GO:0005638 IDA
    nuclear lamina GO:0005652 IBA
    nuclear lamina GO:0005652 IDA
    nuclear lamina GO:0005652 ISO
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nuclear matrix GO:0016363 IDA
    nuclear matrix GO:0016363 ISO
    nuclear membrane GO:0031965 ISO
    nuclear periphery GO:0034399 IDA
 Experiment description of studies that identified Lmnb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lmnb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Actr10  
Co-fractionation Mus musculus
2 Pde4dip  
Affinity Capture-MS Mus musculus
3 Cebpa  
Affinity Capture-MS Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Tnik  
Affinity Capture-MS Mus musculus
6 Tmem173  
Proximity Label-MS Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Mycbp2 105689
Affinity Capture-MS Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Lmna 16905
Affinity Capture-MS Mus musculus
11 Tfe3  
Affinity Capture-MS Mus musculus
12 Cul3  
Affinity Capture-MS Mus musculus
13 Zbtb16  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
14 Spg20  
Co-fractionation Mus musculus
15 Melk  
Co-fractionation Mus musculus
16 Atg12  
Negative Genetic Mus musculus
17 Mettl21e  
Affinity Capture-MS Mus musculus
18 Nanog  
Affinity Capture-MS Mus musculus
19 Fancd2  
Affinity Capture-MS Mus musculus
20 Bccip  
Co-fractionation Mus musculus
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