Gene description for Mcm3
Gene name minichromosome maintenance deficient 3 (S. cerevisiae)
Gene symbol Mcm3
Other names/aliases AL033361
C80350
Mcmd
P1
p1.m
Species Mus musculus
 Database cross references - Mcm3
ExoCarta ExoCarta_17215
Vesiclepedia VP_17215
Entrez Gene 17215
UniProt P25206  
 Mcm3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mcm3
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IBA
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IBA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via break-induced replication GO:0000727 IBA
    DNA unwinding involved in DNA replication GO:0006268 ISO
    DNA replication initiation GO:0006270 IEA
    DNA strand elongation involved in DNA replication GO:0006271 IBA
    premeiotic DNA replication GO:0006279 NAS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
    mitotic DNA replication initiation GO:1902975 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    MCM complex GO:0042555 IBA
    MCM complex GO:0042555 ISO
    MCM complex GO:0042555 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    CMG complex GO:0071162 ISO
    CMG complex GO:0071162 ISS
 Experiment description of studies that identified Mcm3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mcm3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ELL2 22936
Affinity Capture-MS Homo sapiens
2 PMS2  
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 PCGF5  
Affinity Capture-MS Homo sapiens
5 FMNL1 752
Affinity Capture-MS Homo sapiens
6 GAR1 54433
Affinity Capture-MS Homo sapiens
7 SCLY  
Affinity Capture-MS Homo sapiens
8 MCM5 4174
Affinity Capture-MS Homo sapiens
9 SPAG5 10615
Affinity Capture-MS Homo sapiens
10 Atxn1  
Affinity Capture-MS Mus musculus
11 UBA52 7311
Affinity Capture-MS Homo sapiens
12 DGCR8  
Affinity Capture-MS Homo sapiens
13 Atrx  
Co-localization Mus musculus
14 Tfe3  
Affinity Capture-MS Mus musculus
15 ATM 472
Biochemical Activity Homo sapiens
16 GPAM 57678
Affinity Capture-MS Homo sapiens
17 MCM4 4173
Affinity Capture-MS Homo sapiens
18 RCN1 5954
Affinity Capture-MS Homo sapiens
19 Herc2  
Affinity Capture-MS Mus musculus
20 GCC2 9648
Affinity Capture-MS Homo sapiens
21 UBR5 51366
Affinity Capture-MS Homo sapiens
22 TRIM28 10155
Affinity Capture-MS Homo sapiens
23 MCMBP 79892
Affinity Capture-MS Homo sapiens
24 OGT 8473
Affinity Capture-MS Homo sapiens
25 MCM2 4171
Affinity Capture-MS Homo sapiens
26 Nanog  
Affinity Capture-MS Mus musculus
27 AP2A1 160
Affinity Capture-MS Homo sapiens
28 C14orf166 51637
Affinity Capture-MS Homo sapiens
29 Eed  
Affinity Capture-MS Mus musculus
30 EIF2B1 1967
Affinity Capture-MS Homo sapiens
31 MCM6 4175
Affinity Capture-MS Homo sapiens
32 Atg16l1  
Affinity Capture-MS Mus musculus
33 RPP38 10557
Affinity Capture-MS Homo sapiens
34 Hist2h2bb  
Co-fractionation Mus musculus
35 Fancd2  
Affinity Capture-MS Mus musculus
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