Gene description for Rab8a
Gene name RAB8A, member RAS oncogene family
Gene symbol Rab8a
Other names/aliases AA409338
Mel
Species Mus musculus
 Database cross references - Rab8a
ExoCarta ExoCarta_17274
Vesiclepedia VP_17274
Entrez Gene 17274
UniProt P55258  
 Rab8a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab8a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    protein kinase binding GO:0019901 ISO
    small GTPase binding GO:0031267 ISS
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
    protein tyrosine kinase binding GO:1990782 IEA
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    exocytosis GO:0006887 IBA
    vesicle docking involved in exocytosis GO:0006904 IEA
    vesicle docking involved in exocytosis GO:0006904 ISO
    autophagy GO:0006914 IEA
    Golgi organization GO:0007030 ISO
    Golgi organization GO:0007030 ISS
    axonogenesis GO:0007409 ISO
    axonogenesis GO:0007409 ISS
    regulation of autophagy GO:0010506 ISO
    regulation of autophagy GO:0010506 ISS
    endocytic recycling GO:0032456 IBA
    cellular response to insulin stimulus GO:0032869 ISO
    cellular response to insulin stimulus GO:0032869 ISS
    regulation of protein localization GO:0032880 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 IEA
    regulation of long-term neuronal synaptic plasticity GO:0048169 ISO
    Golgi vesicle fusion to target membrane GO:0048210 IEA
    Golgi vesicle fusion to target membrane GO:0048210 ISO
    regulation of protein transport GO:0051223 IEA
    regulation of protein transport GO:0051223 ISO
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to cilium GO:0061512 IMP
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IEA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 ISO
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IBA
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 ISO
    vesicle-mediated transport in synapse GO:0099003 IEA
    vesicle-mediated transport in synapse GO:0099003 ISO
Subcellular Localization
    lysosome GO:0005764 IEA
    endosome GO:0005768 IBA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cilium GO:0005929 IDA
    cilium GO:0005929 IMP
    cilium GO:0005929 ISO
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    synaptic vesicle GO:0008021 IBA
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    postsynaptic density GO:0014069 ISO
    trans-Golgi network transport vesicle GO:0030140 IBA
    trans-Golgi network transport vesicle GO:0030140 IDA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cytoplasmic vesicle membrane GO:0030659 TAS
    phagocytic vesicle membrane GO:0030670 IEA
    cytoplasmic vesicle GO:0031410 ISO
    ciliary basal body GO:0036064 IDA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IBA
    dendritic spine GO:0043197 ISO
    synapse GO:0045202 ISO
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    ciliary membrane GO:0060170 IDA
    ciliary base GO:0097546 IDA
    non-motile cilium GO:0097730 IDA
    non-motile cilium GO:0097730 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rab8a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab8a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SYTL4 94121
Affinity Capture-MS Homo sapiens
2 RAB8A 4218
Affinity Capture-MS Homo sapiens
3 Sstr3  
Proximity Label-MS Mus musculus
4 MICALL2 79778
Affinity Capture-MS Homo sapiens
5 SNCA 6622
Affinity Capture-Western Homo sapiens
6 Mchr1  
Proximity Label-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
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