Gene description for Nefm
Gene name neurofilament, medium polypeptide
Gene symbol Nefm
Other names/aliases NF-M
NF160
NF165
Nef3
Nfm
Species Mus musculus
 Database cross references - Nefm
ExoCarta ExoCarta_18040
Vesiclepedia VP_18040
Entrez Gene 18040
UniProt P08553  
 Nefm identified in sEVs derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Nefm
Molecular Function
    signaling receptor binding GO:0005102 ISO
    structural molecule activity GO:0005198 IEA
    structural constituent of cytoskeleton GO:0005200 IBA
    toxic substance binding GO:0015643 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    microtubule cytoskeleton organization GO:0000226 IGI
    axo-dendritic transport GO:0008088 IMP
    regulation of axon diameter GO:0031133 IMP
    neurofilament bundle assembly GO:0033693 IBA
    neurofilament bundle assembly GO:0033693 ISO
    intermediate filament cytoskeleton organization GO:0045104 IMP
    intermediate filament polymerization or depolymerization GO:0045105 ISO
    intermediate filament bundle assembly GO:0045110 IGI
    neurofilament cytoskeleton organization GO:0060052 IGI
    neurofilament cytoskeleton organization GO:0060052 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoskeleton GO:0005856 ISO
    intermediate filament GO:0005882 IBA
    intermediate filament GO:0005882 ISO
    neurofilament GO:0005883 IDA
    neurofilament GO:0005883 ISO
    postsynaptic density GO:0014069 IDA
    axon GO:0030424 IBA
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    neuromuscular junction GO:0031594 IDA
    neuron projection GO:0043005 IDA
    perikaryon GO:0043204 ISO
    myelin sheath GO:0043209 HDA
    intermediate filament cytoskeleton GO:0045111 ISO
    neurofibrillary tangle GO:0097418 ISO
    postsynaptic intermediate filament cytoskeleton GO:0099160 IBA
    postsynaptic intermediate filament cytoskeleton GO:0099160 IDA
    presynaptic intermediate filament cytoskeleton GO:0099182 IDA
 Experiment description of studies that identified Nefm in sEVs
1
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nefm
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nckap1 50884
Affinity Capture-MS Mus musculus
2 Fxr1 14359
Affinity Capture-MS Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
4 Cyfip1 20430
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Kalrn  
Affinity Capture-MS Mus musculus
8 Sart3  
Co-fractionation Mus musculus
9 Mapt  
Affinity Capture-MS Mus musculus
10 Agap2  
Affinity Capture-MS Mus musculus
11 MAPT  
Reconstituted Complex Homo sapiens
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Ywhaz 22631
Affinity Capture-MS Mus musculus
14 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Nefm is involved
No pathways found





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