Gene description for Nsf
Gene name N-ethylmaleimide sensitive fusion protein
Gene symbol Nsf
Other names/aliases AI316878
AU020090
AU067812
SKD2
Species Mus musculus
 Database cross references - Nsf
ExoCarta ExoCarta_18195
Vesiclepedia VP_18195
Entrez Gene 18195
UniProt P46460  
 Nsf identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nsf
Molecular Function
    SNARE binding GO:0000149 ISS
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IMP
    ATP hydrolysis activity GO:0016887 ISO
    syntaxin-1 binding GO:0017075 IDA
    protein kinase binding GO:0019901 IPI
    syntaxin binding GO:0019905 IDA
    PDZ domain binding GO:0030165 IEA
    PDZ domain binding GO:0030165 ISO
    small GTPase binding GO:0031267 ISO
    D1 dopamine receptor binding GO:0031748 ISO
    ionotropic glutamate receptor binding GO:0035255 IEA
    ionotropic glutamate receptor binding GO:0035255 ISO
    ATP-dependent protein binding GO:0043008 ISO
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 ISS
    metal ion binding GO:0046872 IEA
    cohesin loader activity GO:0061775 IEA
    protein serine/threonine kinase binding GO:0120283 ISO
    ATP-dependent protein disaggregase activity GO:0140545 ISS
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    positive regulation of receptor recycling GO:0001921 IEA
    positive regulation of receptor recycling GO:0001921 ISO
    regulation of receptor internalization GO:0002090 ISO
    chromatin remodeling GO:0006338 IEA
    potassium ion transport GO:0006813 IDA
    intracellular protein transport GO:0006886 IEA
    intracellular protein transport GO:0006886 ISO
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    vesicle-mediated transport GO:0016192 ISO
    regulation of exocytosis GO:0017157 ISO
    protein-containing complex disassembly GO:0032984 ISO
    SNARE complex disassembly GO:0035494 ISS
    Golgi to plasma membrane protein transport GO:0043001 IBA
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    chromatin looping GO:0140588 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi stack GO:0005795 IBA
    Golgi stack GO:0005795 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    dendritic shaft GO:0043198 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Nsf in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nsf
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 Krt35  
Co-fractionation Mus musculus
5 Stau1  
Co-fractionation Mus musculus
6 NAPA 8775
Affinity Capture-Western Homo sapiens
7 Ubc  
Reconstituted Complex Mus musculus
8 Scfd1  
Co-fractionation Mus musculus
9 Snap29  
Co-fractionation Mus musculus
10 Atxn1  
Proximity Label-MS Mus musculus
11 Stx12  
Co-fractionation Mus musculus
12 Thrb  
Affinity Capture-MS Mus musculus
13 Krt27  
Co-fractionation Mus musculus
14 Dlg4  
Affinity Capture-MS Mus musculus
15 Huwe1  
Affinity Capture-MS Mus musculus
16 Ywhaz 22631
Affinity Capture-MS Mus musculus
17 Grin2b  
Affinity Capture-Western Mus musculus
18 Cyfip1 20430
Affinity Capture-MS Mus musculus
19 Grin1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
20 Vamp2  
Co-fractionation Mus musculus
21 Krt82  
Co-fractionation Mus musculus
22 Vamp1  
Co-fractionation Mus musculus
23 Eed  
Affinity Capture-MS Mus musculus
24 Fmr1  
Affinity Capture-MS Mus musculus
25 PRKACB  
Affinity Capture-MS Bos taurus
26 Fancd2  
Affinity Capture-MS Mus musculus
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