Gene description for Plcb3
Gene name phospholipase C, beta 3
Gene symbol Plcb3
Other names/aliases mKIAA4098
Species Mus musculus
 Database cross references - Plcb3
ExoCarta ExoCarta_18797
Vesiclepedia VP_18797
Entrez Gene 18797
UniProt P51432  
 Plcb3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plcb3
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IBA
    phosphatidylinositol phospholipase C activity GO:0004435 IDA
    phosphatidylinositol phospholipase C activity GO:0004435 ISO
    phosphatidylinositol phospholipase C activity GO:0004435 ISS
    calcium ion binding GO:0005509 IEA
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
    molecular function activator activity GO:0140677 ISO
Biological Process
    regulation of systemic arterial blood pressure GO:0003073 ISO
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IDA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IMP
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISS
    phospholipase C-activating serotonin receptor signaling pathway GO:0007208 IEA
    lipid catabolic process GO:0016042 IEA
    phosphatidylinositol catabolic process GO:0031161 ISO
    inositol trisphosphate metabolic process GO:0032957 ISO
    intracellular signal transduction GO:0035556 IEA
    phosphatidylinositol metabolic process GO:0046488 IBA
    phosphatidylinositol metabolic process GO:0046488 ISO
    phosphatidylinositol metabolic process GO:0046488 ISS
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    release of sequestered calcium ion into cytosol GO:0051209 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 ISO
    postsynaptic cytosol GO:0099524 IDA
    postsynaptic cytosol GO:0099524 IEP
 Experiment description of studies that identified Plcb3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plcb3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc9a3r2  
Co-fractionation Mus musculus
2 Slc9a3r1 26941
Co-fractionation Mus musculus
View the network image/svg+xml



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