Gene description for Npepps
Gene name aminopeptidase puromycin sensitive
Gene symbol Npepps
Other names/aliases AAP-S
MP100
Psa
R74825
goku
Species Mus musculus
 Database cross references - Npepps
ExoCarta ExoCarta_19155
Vesiclepedia VP_19155
Entrez Gene 19155
UniProt Q11011  
 Npepps identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Npepps
Molecular Function
    aminopeptidase activity GO:0004177 IEA
    aminopeptidase activity GO:0004177 ISO
    metallopeptidase activity GO:0008237 IEA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IEA
    peptide binding GO:0042277 IBA
    metalloaminopeptidase activity GO:0070006 IBA
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IEA
    peptide catabolic process GO:0043171 IBA
    cellular response to hypoxia GO:0071456 IEA
    cellular response to hypoxia GO:0071456 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IBA
 Experiment description of studies that identified Npepps in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Npepps
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stau1  
Co-fractionation Mus musculus
2 Tnpo1 238799
Co-fractionation Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Cyfip1 20430
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Tia1  
Affinity Capture-MS Mus musculus
8 Ubfd1  
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Pak2 224105
Co-fractionation Mus musculus
11 Dak  
Co-fractionation Mus musculus
View the network image/svg+xml



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