Gene description for Eif2b5
Gene name eukaryotic translation initiation factor 2B, subunit 5 epsilon
Gene symbol Eif2b5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Eif2b5
ExoCarta ExoCarta_192234
Vesiclepedia VP_192234
Entrez Gene 192234
UniProt Q64350  
 Eif2b5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eif2b5
Molecular Function
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 IBA
    translation initiation factor binding GO:0031369 IDA
    translation initiation factor binding GO:0031369 IMP
Biological Process
    ovarian follicle development GO:0001541 ISO
    ovarian follicle development GO:0001541 ISS
    cytoplasmic translational initiation GO:0002183 ISO
    cytoplasmic translational initiation GO:0002183 ISS
    translational initiation GO:0006413 ISO
    translational initiation GO:0006413 ISS
    response to heat GO:0009408 IDA
    response to heat GO:0009408 ISO
    response to heat GO:0009408 ISS
    response to glucose GO:0009749 IDA
    astrocyte development GO:0014002 ISO
    astrocyte development GO:0014002 ISS
    oligodendrocyte development GO:0014003 ISO
    oligodendrocyte development GO:0014003 ISS
    hippocampus development GO:0021766 IEP
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISS
    myelination GO:0042552 ISO
    myelination GO:0042552 ISS
    positive regulation of apoptotic process GO:0043065 IMP
    response to peptide hormone GO:0043434 IDA
    positive regulation of translation GO:0045727 IDA
    positive regulation of translational initiation GO:0045948 ISS
    astrocyte differentiation GO:0048708 ISO
    astrocyte differentiation GO:0048708 ISS
    T cell receptor signaling pathway GO:0050852 ISO
    T cell receptor signaling pathway GO:0050852 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 2B complex GO:0005851 IBA
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
    eukaryotic translation initiation factor 2B complex GO:0005851 ISO
    eukaryotic translation initiation factor 2B complex GO:0005851 ISS
 Experiment description of studies that identified Eif2b5 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif2b5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP1CA 5499
Biochemical Activity Homo sapiens
2 PPP1CC 5501
Biochemical Activity Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here