Gene description for S100a9
Gene name S100 calcium binding protein A9 (calgranulin B)
Gene symbol S100a9
Other names/aliases 60B8Ag
AW546964
BEE22
Cagb
GAGB
L1Ag
MRP14
p14
Species Mus musculus
 Database cross references - S100a9
ExoCarta ExoCarta_20202
Vesiclepedia VP_20202
Entrez Gene 20202
UniProt P31725  
 S100a9 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for S100a9
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IEA
    antioxidant activity GO:0016209 IEA
    calcium-dependent protein binding GO:0048306 IBA
Biological Process
    leukocyte migration involved in inflammatory response GO:0002523 IBA
    leukocyte migration involved in inflammatory response GO:0002523 ISO
    leukocyte migration involved in inflammatory response GO:0002523 ISS
    chronic inflammatory response GO:0002544 IBA
    peptide secretion GO:0002790 IDA
    peptide secretion GO:0002790 IMP
    positive regulation of peptide secretion GO:0002793 IDA
    positive regulation of peptide secretion GO:0002793 IMP
    regulation of translation GO:0006417 IMP
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    apoptotic process GO:0006915 IEA
    positive regulation of neuron projection development GO:0010976 ISO
    astrocyte development GO:0014002 IBA
    astrocyte development GO:0014002 IGI
    peptidyl-cysteine S-nitrosylation GO:0018119 IMP
    actin cytoskeleton organization GO:0030036 IMP
    positive regulation of blood coagulation GO:0030194 IMP
    neutrophil chemotaxis GO:0030593 IBA
    neutrophil chemotaxis GO:0030593 ISO
    neutrophil chemotaxis GO:0030593 ISS
    leukocyte chemotaxis GO:0030595 IMP
    regulation of toll-like receptor signaling pathway GO:0034121 NAS
    autocrine signaling GO:0035425 IBA
    autocrine signaling GO:0035425 ISO
    peptidyl-cysteine S-trans-nitrosylation GO:0035606 ISO
    modulation of process of another organism GO:0035821 ISO
    endothelial cell migration GO:0043542 IBA
    innate immune response GO:0045087 IEA
    regulation of integrin biosynthetic process GO:0045113 IMP
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of inflammatory response GO:0050729 ISS
    regulation of respiratory burst involved in inflammatory response GO:0060264 NAS
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 ISO
    neutrophil aggregation GO:0070488 IBA
    neutrophil aggregation GO:0070488 ISO
    neutrophil aggregation GO:0070488 ISS
    cellular oxidant detoxification GO:0098869 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
Subcellular Localization
    cornified envelope GO:0001533 IDA
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    calprotectin complex GO:1990660 ISO
    S100A9 complex GO:1990662 ISO
 Experiment description of studies that identified S100a9 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for S100a9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r2a 71978
Co-fractionation Mus musculus
2 Strn3  
Co-fractionation Mus musculus
3 Atp1b1 11931
Co-fractionation Mus musculus
4 Ltf  
Co-fractionation Mus musculus
View the network image/svg+xml



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