Gene description for Stxbp1
Gene name syntaxin binding protein 1
Gene symbol Stxbp1
Other names/aliases AI317162
AI326233
MMS10-G
Ms10g
Munc-18a
Munc18-1
N-sec1
Rb-sec1
Sxtbp1
Unc18-1
Unc18h
nsec1
Species Mus musculus
 Database cross references - Stxbp1
ExoCarta ExoCarta_20910
Vesiclepedia VP_20910
Entrez Gene 20910
UniProt O08599  
 Stxbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stxbp1
Molecular Function
    SNARE binding GO:0000149 ISO
    protein binding GO:0005515 IPI
    syntaxin-1 binding GO:0017075 IBA
    syntaxin-1 binding GO:0017075 IDA
    syntaxin-1 binding GO:0017075 IPI
    syntaxin-1 binding GO:0017075 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    syntaxin binding GO:0019905 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    phospholipase binding GO:0043274 IEA
    phospholipase binding GO:0043274 ISO
    molecular adaptor activity GO:0060090 ISO
Biological Process
    platelet degranulation GO:0002576 IEA
    platelet degranulation GO:0002576 ISO
    developmental process involved in reproduction GO:0003006 IEA
    developmental process involved in reproduction GO:0003006 ISO
    intracellular protein transport GO:0006886 IBA
    exocytosis GO:0006887 IMP
    vesicle docking involved in exocytosis GO:0006904 IBA
    vesicle docking involved in exocytosis GO:0006904 IMP
    vesicle docking involved in exocytosis GO:0006904 IMP
    neurotransmitter secretion GO:0007269 IMP
    neuromuscular synaptic transmission GO:0007274 IMP
    axon target recognition GO:0007412 IMP
    regulation of synaptic vesicle priming GO:0010807 IEA
    regulation of synaptic vesicle priming GO:0010807 ISO
    protein transport GO:0015031 IEA
    synaptic vesicle priming GO:0016082 IDA
    synaptic vesicle priming GO:0016082 IMP
    synaptic vesicle maturation GO:0016188 IMP
    negative regulation of protein-containing complex assembly GO:0031333 IEA
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    regulation of vesicle fusion GO:0031338 IEA
    regulation of vesicle fusion GO:0031338 ISO
    synaptic vesicle fusion to presynaptic active zone membrane GO:0031629 TAS
    negative regulation of synaptic transmission, GABAergic GO:0032229 IDA
    response to estradiol GO:0032355 IEA
    SNARE complex assembly GO:0035493 IEA
    SNARE complex assembly GO:0035493 ISO
    positive regulation of mast cell degranulation GO:0043306 IEA
    positive regulation of mast cell degranulation GO:0043306 ISO
    negative regulation of neuron apoptotic process GO:0043524 IMP
    positive regulation of exocytosis GO:0045921 ISO
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IDA
    protein stabilization GO:0050821 ISO
    neuron apoptotic process GO:0051402 IMP
    establishment of localization in cell GO:0051649 IMP
    long-term synaptic depression GO:0060292 IMP
    platelet aggregation GO:0070527 IEA
    platelet aggregation GO:0070527 ISO
    cellular response to type II interferon GO:0071346 IDA
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    presynaptic dense core vesicle exocytosis GO:0099525 IBA
    positive regulation of vesicle docking GO:0106022 IEA
    positive regulation of vesicle docking GO:0106022 ISO
    positive regulation of glutamate secretion, neurotransmission GO:1903296 IEA
    positive regulation of glutamate secretion, neurotransmission GO:1903296 ISO
    regulation of acrosomal vesicle exocytosis GO:2000367 IEA
    regulation of acrosomal vesicle exocytosis GO:2000367 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    secretory granule GO:0030141 IBA
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    platelet alpha granule GO:0031091 IEA
    platelet alpha granule GO:0031091 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    myelin sheath GO:0043209 HDA
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 IDA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
    presynaptic active zone cytoplasmic component GO:0098831 IDA
    extrinsic component of presynaptic membrane GO:0098888 IDA
    extrinsic component of presynaptic membrane GO:0098888 IDA
    extrinsic component of presynaptic membrane GO:0098888 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    presynaptic cytosol GO:0099523 IDA
    presynaptic cytosol GO:0099523 IMP
 Experiment description of studies that identified Stxbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stxbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tuba4a 22145
Co-fractionation Mus musculus
3 Apba1  
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 SNCA 6622
Affinity Capture-MS Homo sapiens
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Tubb2a  
Co-fractionation Mus musculus
8 MTNR1A  
Reconstituted Complex Homo sapiens
9 Agap2  
Affinity Capture-MS Mus musculus
10 MAPT  
Reconstituted Complex Homo sapiens
11 Vamp2  
Co-fractionation Mus musculus
12 Prnp 19122
Affinity Capture-MS Mus musculus
13 Aph1b  
Affinity Capture-MS Mus musculus
14 Aph1a 226548
Affinity Capture-MS Mus musculus
15 Sstr3  
Proximity Label-MS Mus musculus
16 Stx1a  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
17 Htt  
Affinity Capture-MS Mus musculus
18 MTNR1B  
Reconstituted Complex Homo sapiens
19 Kcnma1  
Affinity Capture-MS Mus musculus
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